响应面法优化连钱草三萜酸类成分的超声辅助提取工艺

Optimization of Ultrasonic-assisted Extraction of Triterpene Acids from Glechoma longituba Using Response Surface Methodology

目的:采用响应面法优化超声辅助提取连钱草三萜酸类成分的工艺条件,提高连钱草中三萜酸类成分的得率。方法:以超声时间、液料比及乙醇浓度为主要因素,以齐墩果酸和熊果酸类成分得率为响应值,根据Box-Behnken原理,采用三因素三水平响应面试验设计进行优化。结果:液料比对连钱草中齐墩果酸和熊果酸的提取效果影响最大,其次是超声时间和乙醇浓度。响应面法优化超声辅助提取连钱草中三萜酸类成分的最佳工艺条件为:以88%乙醇为提取溶剂,超声时间为60 min,液料比为78 mL/g,在此条件下齐墩果酸和熊果酸的含量实测值分别为0.8504和1.7367 mg/g。经验证,并与同参数下热浸法、回流法的提取效果相比,超声提取效果最好,提取更为完全。结论:响应面法优化所得的连钱草三萜酸类成分提取工艺稳定可行,可用于连钱草的开发、利用及质量评价。

Objective:To optimize the ultrasound-assisted extraction of triterpene acids from Glechoma longituba by response surface methodology in order to improve extraction efficiency of triterpene acids.Methods:The extraction time,solid-to-liquid ratio and alcohol concentration were used as principal factors with the extraction yield of oleanolic acid and ursolic acid as response value.A regression model for the yield of oleanolic acid and ursolic acid was established using a Box-Behnken design involving the above three factors at three levels.Results:The sequence of different factors on oleanolic acid and ursolic acid contents was solid-to-liquid ratio>extraction time>alcohol concentration,for which the optimal extraction conditions were as follows,extraction time was 60 min,solid-to-liquid ratio was 78 mL/g,and alcohol concentration was 88% as the solvent of supersonic extraction.Under these conditions,the measured contents of oleanolic acid and ursolic acid were 0.8504 and 1.7367 mg/g.After optimization,compared with the hot creep dip and reflux which had same parameters,the ultrasonic extraction was the best.The extraction was more complete.Conclusion:The extraction process of triterpene acids from Glechoma longituba by response surface optimization is stable and feasible,which can be used for the development,utilization and quality evaluation of Glechoma longituba.