叶面喷施水杨酸对当归幼苗生理特性和保护酶活性的影响

Effects of Foliar Spraying with Salicylic Acid on Physiological Characteristics and Antioxidase Activity of Angelica sinensis(Oliv.)Diels

目的:研究叶面喷施水杨酸(SA)对当归幼苗生长和保护酶活性的影响,为当归种子种苗的生产提供科学依据。方法:采用叶面喷施的方法,设置不同处理浓度,考察水杨酸对当归幼苗生物量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性的影响。结果:叶面喷施适宜浓度的水杨酸能促进当归幼苗的生长并提高保护酶活性。其中以0.5mmol·L^-1水杨酸处理的当归幼苗株高和鲜质量为最高,分别为对照组的135.2%和224.6%,超过此浓度,植株生长受到抑制。1.0mmol·L^-1水杨酸处理的当归幼苗,SOD、POD、CAT酶活性分别为对照组的149.1%(7d)、138.2%(7d)、194.7%(5d)。结论:叶面喷施0.5mmol·L^-1的水杨酸能够促进当归幼苗生长,较高浓度的水杨酸对提高当归幼苗SOD、POD、CAT酶活性的效果较好,但会加剧对植株生长的抑制作用。

Objective:The study aims at investigating the influences of exogenous salicylic acid on the growth and antioxidase activity of Angelica sinensis seedlings,and providing a theoretical basis for breeding.Methods:Foliar spraying with a series concentration of salicylic acid were applied.The length and fresh weight,superoxide dismutase,peroxidase and catalase activities of the seedlings were measured after salicylic acid treatments.Results:The growth and antioxidase activity of A.sinensis seedlings could be promoted with appropriate concentration of salicylic acid treatments.The length and fresh weight of the seedlings was increased to 135.2%and 224.6%compared with the control after 0.5 mmol·L^-1 salicylic acid spraying respectively.However,the growth conditions and weights of seedlings were inhibited by increasing concentration of salicylic acid.SOD,POD and CAT activities were promoted to 149.1%(7 d),138.2%(7 d),and 194.7%(5 d)compared with controls respectively under the treatment of 1.0 mmol·L^-1 salicylic acid.Conclusion:0.5 mmol·L^-1 of salicylic acid foliar spraying is benefit for growth condition of seedlings,and relatively higher salicylic acid concentration will be benefit to antioxidase activities promotion,but inhibits the growth condition of A.sinensis seedlings apparently.