疏肝健脾方对肝郁脾虚证模型大鼠下丘脑-垂体-甲状腺轴的影响研究

Effect of Shugan Jianpi recipe on hypothalamus-pituitary-thyroid axis in rats with liver-stagnation and spleen-deficiency syndrome

目的 探查中医肝郁脾虚证甲状腺轴的变化及应用疏肝健脾方的干预作用。方法 利用随机数字表法将40只大鼠随机分为5组,即正常对照组、肝郁脾虚模型组、肝郁脾虚治疗高、中、低剂量组,每组8只。后4组采用慢性束缚应激+过度疲劳+饮食失节的复合方法造模,连续4周,正常对照组不予任何处理。后3组分别在造模第2周周末按照高、中、低剂量(17.98 g/kg、8.99 g/kg、4.50g/kg)给予疏肝健脾方,连续给药14 d,正常对照组、肝郁脾虚模型组给予等容积的蒸馏水。实验第29天,测定血清三磺甲腺原氨酸(Triiodothyronine,T3)、四磺甲腺原氨酸(Thyroxine,T4)、促甲状腺激素(Thyroid Stimulating Hormone,TSH)和促甲状腺激素释放激素(Thyrotropin Releasing hormone,TRH)。实验期间,每天上午8∶30记录24 h饮食量和饮水量。结果 与正常对照组相比,疏肝健脾方干预1、3、5周后肝郁脾虚模型组饮食量明显减少(P<0.05);与肝郁脾虚模型组相比,疏肝健脾方干预1、3、5周后疏肝健脾中剂量组饮食量明显增加(P<0.05),疏肝健脾方干预5周后疏肝健脾高剂量组饮食量明显增加(P<0.05);与正常对照组相比,疏肝健脾方干预1、3、5周后肝郁脾虚模型组饮水量明显减少(P<0.05)。与正常对照组相比,肝郁脾虚模型组大鼠血清T3、T4、TRH均显著降低(P<0.05),TSH显著升高(P<0.05);与肝郁脾虚模型组相比,疏肝健脾中剂量组大鼠血清T3、TRH均显著升高(P<0.05),血清TSH显著降低(P<0.05),血清T4有升高趋势;与肝郁脾虚模型组相比,疏肝健脾低剂量组大鼠T3、TRH显著升高(P<0.05),T4、TSH无明显变化;与肝郁脾虚模型组相比,疏肝健脾高剂量组血清T3、T4、TSH、TRH均无明显变化。结论 肝郁脾虚模型大鼠存在甲状腺功能低下并伴有下丘脑-垂体-甲状腺轴功能的紊乱,疏肝健脾方具有调节甲状腺轴功能紊乱的作用,尤以中剂量组作用最佳。

Objective To explore the changes of the changes of hypothalamus-pituitary-thyroid Axis with liver-stagnation and spleen-deficiency syndrome and the interventional effect of Shugan Jianpi recipe on it.Method 40 rats were randomly divided into normal control group,liver-stagnation and spleen-deficiency model group,the high,middle and low dose groups of Shugan Jianpi recipe with 8 rats in each group by random digital table method.Except for the normal control group,the other groups were made model by chronic restraint stress,excessive fatigue and diet disconnection for 4 weeks.The normal control group was not given any treatment.At the end of the 2nd week,the high,middle and low dose groups of liver stagnation and spleen deficiency were treated with 17.98 g/kg,8.99 g/kg and 4.50 g/kg Shugan Jianpi Recipe respectively for 14 days.Normal control group and liver-stagnation and spleen-deficiency model group were given distilled water of equal volume.On the 29th day of the experiment,the content of serum T3,T4,TSH and TRH were measured.During the experiment,the 24-hour diet and water intake were recorded at 08:30 every day.Result Compared with the normal control group,the diet quantity in the liver stagnation and spleen deficiency model group was obviously reduced(P<0.05).Compared with the liver stagnation and spleen deficiency model group,the diet quantity of the middle dose group of Shugan Jianpi recipe was obviously increased after1、3、5 weeks of intervention with Shugan Jianpi recipe(P<0.05),while the diet quantity of the high dose group of Shugan Jianpi recipe was obviously increased after 5 weeks of intervention with Shugan Jianpi recipe.Compared with the normal control group,the water consumption was decreased,the content of serum T3,T4 and TRH were significantly decreased(P<0.05),and TSH was significantly increased(P<0.05)in the liver stagnation and spleen deficiency model group.Compared with the liver stagnation and spleen deficiency model group,the content of the serum T3 and TRH in the low and middle dose group of Shugan Jianpi recipe was significantly increased(P<0.05)and the content of the serum TSH in the middle dose group of Shugan Jianpi recipe was significantly decreased(P<0.05).Compared with the liver stagnation and spleen deficiency model group,the content of serum T4 had an increasing trend in the middle dose group of Shugan Jianpi recipe.There was no significant difference in the content of serum T4 and TSH between the low dose group of Shugan Jianpi recipe and the liver stagnation and spleen deficiency model group.There was no significant difference in the content of serum T3,T4,TSH,TRH between the high dose group of Shugan Jianpi recipe and the liver stagnation and spleen deficiency model group.Conclusion There are hypothyroidism and hypothalamus-pituitary-thyroid axis dysfunction in rats with liver stagnation and spleen deficiency.Shugan Jianpi recipe can regulate the dysfunction of thyroid axis,especially in the treatment of the middle dose of Shugan Jianpi recipe.