黄花牛耳朵和牛耳朵转录组分析及苯乙醇苷类化合物合成相关基因的挖掘

Transcriptome Analysis of Primulina lutea and Primulina eburnea and Gene Mining Related to Synthesis of Phenylglycosides

目的:挖掘苯乙醇苷类物质在黄花牛耳朵及牛耳朵叶片中合成的关键基因。方法:对两种植物的叶片进行转录组测序,unigene在公共数据库注释,对差异表达基因进行富集分析。结果:两种植物的unigene以短片段为主,GO及KEGG分类结果都显示两种植物的基因表达谱相似,但叶片的次生物质合成可能存在差异,2 735个差异表达基因被富集到121条pathway中,与苯乙醇苷类产物代谢相关的通路有3条。结论:理清了黄花牛耳朵及牛耳朵苯乙醇苷类物质合成代谢通路,并注释了大量关键酶的基因,可为后续苯乙醇苷类化合物生物合成关键基因的克隆、功能验证以及次生代谢途径解析提供基础数据。

Objective:To study the key genes synthesized from phenylglycosides in the leaves of Primulina lutea and Primulina eburnea.Methods:Transcriptome sequencing was performed on the leaves of two plants,unigenes were annotated by the public database and enrichment analysis was carried out for differentially expressed genes.Results:Unigenes of two plants was mainly short sequences,GO and KEGG classification showed that the gene expression profiles were similar in two plants,but the blade secondary substances synthetic were different,2 735 differentially expressed genes were enriched to 121 metabolic pathway,and metabolic pathways had three related phenylethanoid glycosides.Conclusion:The anabolic pathway of phenylethanoid glycosides in Primulina lutea and Primulina eburnea is clarified,and a large number of key enzyme genes are annotated,which provides basic data for the cloning,functional verification and secondary metabolic pathway analysis of key genes for biosynthesis of phenylethanoid glycosides.