调气活血养阴方对慢性萎缩性胃炎大鼠壁细胞超微结构的影响

Effects of Tiaoqi Huoxue Yangyin Fang on oxyntic cell ultrastrucutre in rats with chronic atrophic gastritis

目的观察调气活血养阴方对慢性萎缩性胃炎(CAG)大鼠壁细胞超微结构的影响,从微观角度探索调气活血养阴方延缓或阻断CAG向胃癌转化的机制。方法将26只雄性大鼠随机分为正常组(10只)和造模组(16只),造模组采用四因素联合法造模,即N-甲基-N′-硝基-N-亚硝基胍(MNNG)、雷尼替丁饲料、饥饱失常和水杨酸钠灌胃,造模第26周,正常组和造模组各随机抽检7只大鼠,经胃组织病理染色确认造模组CAG造模成功后,将造模组剩余9只大鼠随机分为模型组、胃复春组和调气活血养阴方组,其中胃复春组以0.432 g/(kg·d)的剂量给予胃复春混悬液灌胃,调气活血养阴方组以1.838 g/(kg·d)的剂量给予调气活血养阴方灌胃治疗12周,正常组和模型组给予相同体积的高温高压灭菌水灌胃。第26周和39周取材,制作胃组织电镜标本,在透射电镜下观察壁细胞的超微结构。结果造模第26周,正常组大鼠壁细胞处于静息状态,造模组壁细胞处于分泌状态,正常组和造模组的线粒体数目差异无统计学意义,线粒体结构也无明显差异。第39周干预后,正常组大鼠线粒体结构异常的比例低于模型组(P<0.01)。模型组大鼠壁细胞处于分泌静止状态,线粒体数目最少,结构破坏严重。经调气活血养阴方治疗后,壁细胞多处于分泌状态,线粒体数量较模型组增多(P<0.01),结构异常的线粒体数目减少(P<0.01)。结论调气活血养阴方可能通过改善壁细胞超微结构、促进线粒体的能量代谢、调节壁细胞的分泌状态、增强其泌酸功能而起到延缓或阻断CAG进展的作用。

Objective To observe the influence of Tiaoqi Huoxue Yangyin Fang(Qi-regulating, blood-activating and yin-nourishing formula, THYF) on oxyntic cell ultrastructure in rats with chronic atrophic gastritis(CAG) and explore its mechanism of delaying or blocking the transformation to gastric cancer from a microscopic perspective. Methods 26 male rats were randomly divided into normal group(n =10) and model group(n =16). The model group was made by using tetra-treatment method, namely N-methyl-N-Nitro-nitrosoguanidine(MNNG), ranitidine, sodium salicylate, and starvation. At the 26thweek of modeling,7 rats were sacrificed in each group to perform pathological staining of stomach tissue and confirm CAG model. The remaining 9 rats in the model group were randomly divided into three groups: the model group, Weifuchun(WFC) group and THYF group, and the WFC group was orally given Weifuchun decoction at the dose of 0.432 g/(kg·d), and the THYF group was given THYF decoction orally at the dose of 1.838 g/(kg·d) for 12 weeks. The normal group and model group were given the same volume of sterilized water(under high temperature and pressure). At the 26th and 39th week, electron microscopic specimens of gastric tissue were made to observe the ultrastructure of gastric oxyntic cells under the transmission electron microscope(TEM). Results At the 26th week of modeling,the oxyntic cells in the normal group and model group were separated in resting and secretion state. There was no significant difference in the number and structure of mitochondria between the two groups. At the 39th week,the proportion of mitochondrial structural abnormalities in the normal group was lower than that in model group(P<0.01). The parietal cells in the model group were in a static state of secretion,while the number of mitochondria was the least and the structure was the most damaged. After treatment by THYF, most parietal cells were in secretion state;the number of mitochondria increased(P<0.01), and the number of mitochondrial abnormalities decreased(P<0.01), compared with the model group. Conclusion THYF could improve the ultrastructure of oxyntic cells,thus promoting the energy metabolism of mitochondria and regulating the secretory state,ehancing the acid-secreting function, and eventually delaying or blocking the progress of CAG.