摘要
While being one of the world's most important crops,maize ( Zea mays L.) is still difficult to regenerate in tissue culture which severely limits its improvement by genetic engineering.Currently,immature zygotic embryos provide the predominantly used material for regeneration and transformation.However,the procedures involved are often laborious,time-consuming and season-dependent.Here,we further improved an efficient tissue culture and plant regeneration system that uses maize leaf segments of young seedlings as an alternative explant source.Embryogenic calli were evaluated by morphology,proliferation and regeneration capacity.All these indicated that seedling-derived leaf materials have the potential to replace immature embryos for tissue culture and regeneration.
While being one of the world’s most important crops, maize(Zea mays L.) is still difficult to regenerate in tissue culture which severely limits its improvement by genetic engineering. Currently, immature zygotic embryos provide the predominantly used material for regeneration and transformation. However, the procedures involved are often laborious, time-consuming and season-dependent. Here, we further improved an efficient tissue culture and plant regeneration system that uses maize leaf segments of young seedlings as an alternative explant source. Embryogenic calli were evaluated by morphology, proliferation and regeneration capacity. All these indicated that seedling-derived leaf materials have the potential to replace immature embryos for tissue culture and regeneration.
