摘要
[Objectives]This study was conducted to investigate the analgesic effects and acute toxicities of Bidens alba (L.) DC.[Methods]The alcohol extract of B.alba (L.) DC was extracted and separated with petroleum ether and chloroform successively.The acute toxicities of the two extracts on mice were measured,and then the analgesic effects were measured with writhing pain model induced by acetic acid.[Results]No mice died when the crude dosages of B.alba (L.) DC from petroleum ether extract and chloroform extract were 5 016 and 5 100 mg/kg,respectively.When the petroleum ether extract was 60.0 mg/kg,the percentage of twisted mice induced by acetic acid was 40%,the analgesic rate was 77.5%,and the time of the first writhing was (294.0±165.8) s;when the chloroform extract was 20.0 mg/kg,the percentage of twisted animals was 55.6%,the analgesic rate was 51.5%,and the time of the first writhing was (273.8 ±153.4) s;and when the chloroform extract was 4.0 mg/kg,the percentage of twisted animals was 40%,and the analgesic rate was 62.1%,and the time of the first writhing was (370.6±231.3) s.[Conclusions]The petroleum ether extracts and chloroform extracts of B.alba (L.) DC have good analgesic effects and no acute toxicities.
[Objectives] This study was conducted to investigate the analgesic effects and acute toxicities of Bidens alba(L.) DC. [Methods] The alcohol extract of B. alba(L.) DC was extracted and separated with petroleum ether and chloroform successively. The acute toxicities of the two extracts on mice were measured, and then the analgesic effects were measured with writhing pain model induced by acetic acid. [Results] No mice died when the crude dosages of B. alba(L.) DC from petroleum ether extract and chloroform extract were 5016 and 5100 mg/kg, respectively. When the petroleum ether extract was 60.0 mg/kg, the percentage of twisted mice induced by acetic acid was 40%, the analgesic rate was 77.5%, and the time of the first writhing was(294.0±165.8) s; when the chloroform extract was 20.0 mg/kg, the percentage of twisted animals was 55.6%, the analgesic rate was 51.5%, and the time of the first writhing was(273.8 ±153.4) s; and when the chloroform extract was 4.0 mg/kg, the percentage of twisted animals was 40%, and the analgesic rate was 62.1%, and the time of the first writhing was(370.6±231.3) s. [Conclusions] The petroleum ether extracts and chloroform extracts of B. alba(L.) DC have good analgesic effects and no acute toxicities.
基金
Supported by Special Fund for Scientific and Technological Development of Guangdong Province(2017A020225023)
President Fund of Guangdong Academy of Agricultural Sciences(201627)
Special Research Fund for Medical and Health of Huadu District of Guangzhou City(17-HDWS-037)
