86份酸枣种质资源筛选和遗传多样性分析

Screening and Genetic Diversity Analysis of 86 Sour Jujube Germplasm Resources

[目的]综合酸枣种质资源主要农艺性状评价和遗传多样性分析结果,筛选优良酸枣种质资源。[方法]对86份酸枣种质资源的15个农艺性状进行表型鉴定,并利用9个ISSR标记进行遗传多样性分析。[结果]通过对15个农艺性状综合评价,从86份酸枣种质资源中筛选出5份优良种质资源。9对ISSR引物共扩增出20条多态性条带,多态性信息量(polymorphisminformationcontent,PIC)为0.60~0.81,平均值为0.70。在遗传系数0.57的水平上86份酸枣种质资源被分为两大类,其中79份种质资源聚为A类,7份种质资源聚为B类。[结论]该研究为酸枣种质资源分类、鉴定和新品种选育等提供理论依据。

[Objective] The main agronomic traits and genetic diversity analysis results were studied to screen the excellent sour jujube germplasm resources.[Method] 15 agronomic traits of 86 sour jujube germplasm resources were identified,and 9 inter-simple sequence repeat (ISSR) primers were used for genetic diversity analysis.[Result] Through the comprehensive evaluation of 15 agronomic traits,5 excellent germplasm resources were selected from 86 sour jujube individuals.A total of 20 polymorphic bands were amplified by 9 ISSR primers.The polymorphism information content (PIC) ranged from 0.60 to 0.81 with an average of 0.70.Clustering analysis showed that the 86 sour jujube germplasm resources could be divided into 2 groups at the genetic similarity coefficient of 0.57,79 sour jujube individuals were grouped into class A and 7 sour jujube individuals were grouped into class B.[Conclusion] This study provides a useful basis for the classification,identification and variety selection of sour jujube germplasm resources.