DNA条形码在本溪洋湖沟水生昆虫鉴定中的应用

Identificatioin of Aquatic Insects in Yanghugou Based on DNA Barcode

[目的]通过调查本溪市洋湖沟水生昆虫的种类,评价洋湖沟地区太子河源头的水质情况。[方法]2017年7月对洋湖沟水生昆虫进行了采集,基于EPT丰富度、采用DNA条形码及形态学特征对洋湖沟所采水生昆虫进行鉴定。[结果]共得到11个种14条样品序列,其隶属于3目11科:蜉蝣目3种,襀翅目4种,毛翅目4种。[结论]鉴定结果显示,洋湖沟地区EPT水生昆虫种类较多,水质清洁,达到国家I类标准。

[Objective]In order to investigate the species of aquatic insects in Yanghugou,Benxi,evaluate the water quality of the source of the Taizi River in Yanghugou area.[Method]Aquatic insects of Yanghugou were collected in July 2017. Based on the EPT richness,DNA barcode method and morphological features,the aquatic insects collected in Yanghugou were identified.[Result]A total of 14 sequences were obtained,totaling 11 species,belonging to 3 orders and 11 families. Among them,there are 3 species of the Ephemeroptera;4 species of the Plecoptera;and 4 species of the Trichoptera.[Conclusion]The identification results show that there are many aquatic insects in the Yanghugou area,and the water quality is clean. It has reached the national class I standard.