磷酸三(1,3-二氯二丙基)酯(TDCPP)对斑马鱼胚胎急性毒性研究

Study on Acute Toxicity of Tris (1,3-dichloro-2-propyl) Phosphate (TDCPP)to Zebra Fish Embryo

磷酸三(1,3-二氯二丙基)酯(TDCPP)是环境中广泛存在的新兴持久性有机污染物。实验将受精1h后的斑马鱼胚胎暴露于TDCPP(0,5μg/L,20μg/L,80μg/L和180μg/L)至受精后168h,在特定时间点观察并记录胚胎/幼鱼的存活率(24~120haf)、孵化率与畸形率(72haf)、心率(48haf,84haf)、体重与体长(168haf)。结果显示:与对照组相比,急性暴露TDCPP使斑马鱼胚胎/幼鱼的存活率与孵化率降低、畸形率增加、心率以及体长受到显著抑制,表明TDCPP对斑马鱼胚胎存在显著的急性毒性,并且这种影响随着暴露液TDCPP浓度的增大更为明显。

Tris (1,3-dichloro-2-propyl) phosphate(TDCPP) is a new organic long-lasting pollutant widely existing in the environment.This experiment exposed zebrafish embryos (1 hour after fertilization) to TDCPP(0,5 μg/L,20 μg/L,80 μg/L and 180 μg/L) to 168 hours after fertilization.During the experiment,survival rate (24~120 haf),incubation rate and malformation rate (72 haf),heart rate (48 haf,84 haf),weight and body length (168 haf) were observed and recorded at a specific time.The results show that acute toxic exposure to TDCPP have an obvious impact on embryonic development.Compared with the control group,the survival rate and incubation rate decreased,the malformation rate increased,and the heart rate and body length was significantly inhibited,which indicates that TDCPP had significant acute toxicity to zebra fish embryos,and the impact is more serious with the increase of TDCPP concentration.