非小细胞肺癌患者的肿瘤转移与肿瘤浸润的CD8^+T细胞数量负相关

Tumor metastasis in patients with non-small cell lung cancer is inversely correlated with the number of tumor-infiltrating CD8^+ T cells

目的分析非小细胞肺癌(NSCLC)患者原位肿瘤中浸润T细胞数量与临床转移及患者临床预后的关系。方法选取肺癌组织共140例(其中发生转移的43例),采用免疫组织化学染色法检测肿瘤组织中CD4^+ T细胞和CD8^+ T细胞在肿瘤组织的浸润情况,对比未转移和转移患者肿瘤组织CD4^+T细胞和CD8^+ T细胞的浸润数量,采用生存分析对比不同CD4^+ T细胞和CD8^+ T细胞浸润数量对患者临床预后的影响。结果两组患者肿瘤组织CD4^+ T细胞比例差异不大,转移组患者肿瘤组织CD8^+ T细胞数量显著低于转移组,且未转移组CD8/CD4比例显著高于转移组, CD8^+ T细胞数量多和CD8/CD4比例高的患者总体生存率显著优于CD8^+ T细胞数量少和CD8/CD4比例低的患者。结论出现转移的NSCLC患者肿瘤组织CD8^+ T细胞浸润数量、 CD8/CD4比例均显著下降,与NSCLC转移患者预后差密切相关。

Objective To analyze the relationship between the number of invasive T cells in in situ tumors and the clinical metastasis and prognosis in patients with non-small cell lung cancer(NSCLC). Methods A total of 140 lung cancer patients(including 43 cases with metastasis) were selected to observe the infiltration state of CD4^+ T cells and CD8^+ T cells in tumor tissues by immunohistochemical staining. The infiltration of CD4^+ T cells and CD8^+ T cells were compared between non-metastatic and metastatic patients. The effects of different infiltration levels of CD4^+ T cells and CD8^+ T cells on clinical prognosis were analyzed. Results The proportion of CD4^+ T cells in the tumor tissues of the two groups was not significantly different. The number of CD8^+ T cells in the metastatic group was significantly lower than that in the metastatic group, and the ratio of CD8/CD4 in the non-metastasis group was remarkably higher than that in the metastatic group. Patients with high CD8^+ T cell infiltration level or high ratio of CD8/CD4 have a significantly better overall survival rate than the patients with low CD8^+ T cell infiltration level or low ratio of CD8/CD4. Conclusion The number of CD8^+ T cell infiltration and CD8/CD4 ratio in the tumor tissues of patients with metastatic NSCLC are significantly reduced, which is closely related to the poor prognosis of patients with NSCLC metastasis.