不同产地姜黄属植物中姜黄素类成分及矿质元素分析

Analysis of Curcuminoids and Mineral Elements in Curcuma from Different Habitats

以福建泉州地区种植的12个姜黄(Curcumalonga)和莪术(Curcumazedoaria)种质的成熟根茎为试材,采用正交设计法优化姜黄素(CCM)、单脱甲氧基姜黄素(DMC)和双脱甲氧基姜黄素(BDMC)的提取工艺条件,比较不同产地姜黄属种质的3种姜黄素类化合物和16种矿质元素的含量。结果表明,姜黄素类化合物提取工艺为:100%甲醇, 1:5 (g·mL-1)固液比,超声提取2分钟,提取率达到最高值。CCM、DMC和BDMC的最大吸收波长分别为424、418及414nm;回归方程分别为Y=0.170 3X+0.024 3 (CCM), Y=0.173 8X+0.041 (DMC), Y=0.140 6X+0.051 4 (BDMC)。GY03种质中CCM、DMC和BDMC含量分别为1.23%、1.22%与1.50%,总姜黄素类含量最高,达3.95%; GY01种质的总姜黄素次之(为3.82%); GY03和GY01可在栽培地推广种植; 12个姜黄属种质的16种矿质元素中,以Mg、Mn、Al、Fe和Zn含量较为丰富。

In this study, the mature rhizomes of 12 germplasms of Curcuma longa and C. zedoaria from different origins cultivated in Fujian, Quanzhou, were used as experimental materials. The orthogonal design method was used to optimize the extraction of curcumin (CCM), demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC), and the content of 3 curcuminoids and 16 mineral elements in different germplasms were compared. The extraction rate peaked with the following extraction process: 100% methanol as solvent, 1:5 (g·mL-1) solid-liquid ratio, and ultrasonic extraction for 2 min. The maximum absorption wavelengths for CCM, DMC and BDMC were 424, 418 and 414 nm, respectively. The linear equations for the 3 curcuminoids were Y=0.170 3X+0.024 3 (CCM), Y=0.173 8X+0.041 (DMC), and Y=0.140 6X+0.051 4 (BDMC). The content of CCM, DMC and BDMC in GY03 was 1.23%, 1.22% and 1.50%, respectively, and that of total curcumin was the highest, 3.95%;The content of total curcumin in GY01 was next, up to 3.82%. GY03 and GY01 can be promoted in cultivated land as excellent breeding material. Among the 16 mineral elements detected in 12 turmerics, the contents of Mg, Mn, Al, Fe, and Zn were relatively abundant.