迟缓爱德华氏菌耐药性分析研究

Analysis of antibiotic resistance of Edwardsiella tarda

目的分析来自不同地区和宿主的迟缓爱德华氏菌(Edwardsiella tarda, Et)耐药特性及抗性基因。方法采用VITEK 2 Compact全自动分析系统,利用VITEK 2 Compact AST-GN13(22095)药敏鉴定卡对23株Et进行药敏试验;通过实时荧光PCR法检测抗性基因。结果manE291、ET-0711059、L49231、DT这4株Et对复方新诺明耐药,其中ET-0711059还对包括氨苄西林、庆大霉素等在内的其余7种抗生素耐药,对环丙沙星处于中介耐药。23株Et经氨基糖苷类、四环素类、磺胺类、喹诺酮类、大环内酯类等30种抗性基因检测,16%Et含有氨基糖苷类ant(3”)-I抗性基因及磺胺类sul1抗性基因;另有16%Et含磺胺类sul2抗性基因;40%Et含四环素类tetA抗性基因。所有耐药菌株均为鱼源株,6株人源菌株不表现耐药。结论所检测的Et菌株除了1株多重耐药,其余菌株的耐药性并不突出,主要表现为对复方新诺明的耐药。在检测的Et菌株中分布有一定比例的抗性基因,但和耐药性并不完全相关。

Objective To analyze the characteristics of antibiotic resistance and antibiotic resistance genes in Edwardsiella tarda (Et) from different regions and hosts. Methods Using VITEK 2 Compact automatic analysis system, totally 23 strains of Et were tested by VITEK 2 Compact AST-GN13 (22095) susceptibility identification card. Resistance genes were detected by real-time fluorescence PCR. Results The 4 strains of ET, manE291, et-0711059, L49231, DT, were resistant to compound xinnomin, and et-0711059 was also resistant to the other seven antibiotics including ampicillin and gentamicin, with intermediate resistance to ciprofloxacin. Totally 23 strains of Et were tested for 30 resistance genes including aminoglycosides, tetracyclines, sulfonamides, quinolones and macrolides, 16% of them contained aminoglycoside ant(3”)-I and sulfonamide sul1, and 16% of them contained sulfonamide sul2;40% of them contained tetracycline tetA resistance gene. All antibiotic resistant strains were fish-derived strains, and 6 human-derived strains showed no antibiotic resistance. Conclusion In addition to one strain of multi-antibiotic resistance, the resistance of the other strains is not prominent, mainly manifest as resistance to compound neotamine. A certain proportion of resistance genes are distributed in the tested strains, but they are not completely related to antibiotic resistance.