大豆β-Conglycinin对鱼类健康的影响及分离方法

Effects of Soybean β-Conglycinin on Fish Health and Methods of Separation

大豆抗原蛋白是限制大豆蛋白在水产饲料中广泛应用的主要因素。β-伴大豆球蛋白(β-Conglycinin)是免疫原性最强的大豆抗原蛋白,饲料中β-Conglycinin含量过高会导致鱼类生长速度下降、消化酶活力下降、肠道组织发生损伤,甚至死亡。本文概述β-Conglycinin的理化性质、对鱼类生长性能、肠道组织、消化酶活力、非特异性免疫指标和抗氧化能力的影响、分离提纯方法及去除工艺,为进一步研究大豆抗原蛋白的致敏机理提供参考,同时为合理开发利用大豆蛋白源提供依据。

Soybean antigen protein is a major factor limiting the widespread use of soy protein sources in aquafeeds.β-Conglycinin is the most immunogenic soybean antigen protein. The high content of β-Conglycinin in fish feed can result in decreased fish growth rate and digestive enzyme activity, intestinal damage, and even death. This paper summarized the physicochemical properties, separation and purification methods, removal process of β-Conglycinin and its effects on fish growth performance, intestinal tissue, digestive enzyme activity, non-specific immune index and antioxidant ability. This study provides reference for further research on the sensitisation mechanism of soybean antigenic protein, reasonable development and utilization of soybean protein source.