表面增强红外吸收光谱——表面敏感的原位免标记光谱电化学技术

Surface-Enhanced Infrared Absorption Spectroscopy-Surface Sensitive in Situ Label-Free Spectroelectrochemistry

表面增强红外吸收光谱(尤其衰减全反射表面增强红外吸收光谱)是一种超灵敏的红外光谱技术,能够实现亚单层膜水平的表面选择性探测.由于增强基底可同时作为工作电极实现电化学调制,衰减全反射表面增强红外吸收光谱是一种表面敏感的原位免标记光谱电化学技术.本文首先简要介绍了表面增强红外吸收光谱的基本原理和技术特点,之后通过代表性研究工作着重介绍近年衰减全反射表面增强红外吸收光谱电化学的应用和发展,最后展望了表面增强红外光谱所面临的挑战和潜在的研究方向.

Surface-enhanced infrared absorption spectroscopy(SEIRAS), especially in attenuated total reflectance(ATR) mode,taking advantages of surface-enhancement and near-field optical effect of enhancing substrate, is a ultra-sensitive infrared spectroscopy, which could achieve surface-selected detection at a sub-monolayer level. Since the enhancing substrate could simultaneously serve as a working electrode, ATR-SEIRAS is a readily surface-sensitive in situ label-free spectroelectrochemistry technique. With the advantages of small influence from metal species on enhancement effect, good potential reversibility of spectra,simple surface selection rule and sensitivity to polar molecules, ATR-SEIRAS has been widely applied in the fields of orientation analysis and species identification of interfacial adsorbed molecules, interfacial catalytic reaction mechanism elucidation, and interfacial molecular interaction study. In recent years, ATR-SEIRAS has been demonstrated to be a powerful tool in biomolecule analysis, and great progress has been made. Therefore, this review briefly introduces the basic principle and basic technical characteristics of SEIRAS, and then emphasizes the application and development of ATR-SEIRAS in protein structure-function study, artificial biomimetic membrane research, whole cell analysis, as well as electrocatalytic interface probing, through discussing several representative research works. Finally, some perspectives for future development of SEIRAS are discussed.