温州地区畲族人群15个STR基因座遗传多态性

Genetic Polymorphism of 15 STR Loci of She-ethnic Population in Wenzhou Area

目的调查温州地区畲族人群15个STR基因座的遗传多态性,充实温州地区畲族人群群体遗传学数据。方法应用AmpFLSTR■ Identifiler■ Plus试剂盒对温州地区108名畲族无关个体血样进行PCR扩增,经3500xL型遗传分析仪电泳检测,用GeneMapperID-X软件进行等位基因分型。结果共检出134个等位基因,基因频率在0.005~0.546之间。除了D2S1338基因座外,其余基因座基因型频率均符合Hardy-Weinberg平衡(P>0.05)。温州畲族群体期望杂合度为0.599~0.886,个体识别率为0.762~0.968,多态信息含量为0.540~0.870,非父排除率为0.293~0.735;累积随机匹配概率为7.19×10^-17;累积个体识别力为0.99999999999999992807;累积非父排除概率为0.9999913。结论15个STR位点在温州畲族人群中具有较好的遗传多态性,可用于温州地区畲族群体的个体识别和亲权鉴定。

Objective To investigate the genetic polymorphism and frequencies of 15 short tandem repeats (STRs) loci of She-ethnic population in the area of Wenzhou,Zhejiang province.Methods DNA samples were collected from 108 unrelated She-ethnic individuals in Wenzhou.15 STR loci (D8S1179,D21S11,D7S820,CSF1PO,D3S1358,TH01,D13S317, D16S539,D2S1338,D19S433,vWA,TPOX,D18S51,D5S818,FGA) were amplified with Identifiler plus kit,with the PCR products being analyzed by 3500XL Genetic Analyzer and genotyped through GeneMapper ID-X software.The genetic data was statistically parsed into Modified-powerstats software.Results 134 alleles were identified of frequencies ranging from 0.005~0.546.The distributions of the 14 STR loci were in accordance with Hardy-Weinberg equilibrium except the D2S1338 (P>0.05).The expected heterozygosity (He) were 0.599 ~ 0.886,together with the discrimination power (DP) being 0.762 ~ 0.968,the polymorphic information content (PIC) spanning 0.540 ~ 0.870,the probability of paternity exclusion (PE) bestriding 0.293 ~ 0.735,the total discrimination power (TDP) being 0.999 999 999 999 999 928 07,and the cumulative probability of paternity exclusion (CEP) showing 0.9999913.Conclusion The genetic polymorphism of 15 STR in Wenzhou’s She-ethnic population is of great significance for human population genetics and forensic paternity.