有氧运动和谷氨酰胺对二型糖尿病大鼠氧化应激及相关因子表达的影响

Effects of aerobic exercise and glutamine on oxidative stress and expression of related factors in type 2 diabetic rats

目的:探讨有氧运动和谷氨酰胺(Gln)对二型糖尿病(T2MD)大鼠抗氧化应激及炎性因子的影响。方法:用链脲佐菌素(STZ)诱导糖尿病大鼠模型,将成年雄性SD大鼠50只,6周龄,随机分为5组(n=10):包括安静对照组(N)、糖尿病对照组(D)、糖尿病施加有氧运动组(DE)、糖尿病施加谷氨酰胺组(DG)、糖尿病施加有氧运动+谷氨酰胺组(DEG)。6周后,检测干预后糖尿病大鼠糖脂代谢、抗氧化应激及炎性因子等相关指标,并探讨影响炎症反应的可能机制。结果:与N组相比,D组大鼠血清MDA、血糖、TC、TG、胰岛素、瘦素、TNF-α水平均明显升高(P<0.01),血清中SOD、GSH-Px、脂联素、HDL-C的水平均明显降低(P<0.01);与D组相比,三个干预组血清中MDA、血糖、TC、TG、胰岛素、瘦素、TNF-α水平降低,血清中HDL-C、SOD、GSH-Px、脂联素的水平均明显升高(P<0.05,P<0.01),且两者联合对上述指标的改善作用更为明显(P<0.01)。结论:有氧运动和Gln均能缓解糖尿病大鼠糖脂代谢及紊乱、氧化应激损伤及炎症反应,两者联合对其作用优于单一作用。

Objective:To investigate the effects of aerobic exercise and glutamine(Gln)on anti-oxidative stress and inflammatory factors in type 2 diabetes mellitus(T2MD)rats.Methods:Diabetic rat model was induced by streptozotocin(STZ).Fifty 6-week old male SD rats were randomly divided into 5 groups(n=10),including quiet control group(N),diabetes control group(D),diabetic aerobic exercise group(DE),diabetic glutamine group(DG)and diabetic aerobic exercise glutamine group(DEG).After 6 weeks,the related indicators of glucose and lipid metabolism,anti-oxidative stress and inflammatory factors in diabetic rats were detected,and the possible mechanism affecting inflammatory response were explored.Results:Compared with group N,the levels of serum malondialdehyde(MDA),blood glucose,total cholesterol(TC),triglyceride(TG),insulin,leptin and tumor necrosis factor-α(TNF-α)in group D were increased significantly(P<0.01).Compared with group D,serum levels of MDA,blood glucose,TC,TG,insulin,leptin and TNF-αin three intervention groups were decreased significantly,while the levels of SOD,GSH-Px and adiponectin were increased,and the combined effect was more obvious(P<0.01).Conclusion:Both aerobic exercise and Gln can relieve the glucose and lipid metabolism and disturbance,oxidative stress injury and inflammation in diabetic rats.