摘要
目的:探讨有氧运动和谷氨酰胺(Gln)对二型糖尿病(T2MD)大鼠抗氧化应激及炎性因子的影响。方法:用链脲佐菌素(STZ)诱导糖尿病大鼠模型,将成年雄性SD大鼠50只,6周龄,随机分为5组(n=10):包括安静对照组(N)、糖尿病对照组(D)、糖尿病施加有氧运动组(DE)、糖尿病施加谷氨酰胺组(DG)、糖尿病施加有氧运动+谷氨酰胺组(DEG)。6周后,检测干预后糖尿病大鼠糖脂代谢、抗氧化应激及炎性因子等相关指标,并探讨影响炎症反应的可能机制。结果:与N组相比,D组大鼠血清MDA、血糖、TC、TG、胰岛素、瘦素、TNF-α水平均明显升高(P<0.01),血清中SOD、GSH-Px、脂联素、HDL-C的水平均明显降低(P<0.01);与D组相比,三个干预组血清中MDA、血糖、TC、TG、胰岛素、瘦素、TNF-α水平降低,血清中HDL-C、SOD、GSH-Px、脂联素的水平均明显升高(P<0.05,P<0.01),且两者联合对上述指标的改善作用更为明显(P<0.01)。结论:有氧运动和Gln均能缓解糖尿病大鼠糖脂代谢及紊乱、氧化应激损伤及炎症反应,两者联合对其作用优于单一作用。
Objective:To investigate the effects of aerobic exercise and glutamine(Gln)on anti-oxidative stress and inflammatory factors in type 2 diabetes mellitus(T2MD)rats.Methods:Diabetic rat model was induced by streptozotocin(STZ).Fifty 6-week old male SD rats were randomly divided into 5 groups(n=10),including quiet control group(N),diabetes control group(D),diabetic aerobic exercise group(DE),diabetic glutamine group(DG)and diabetic aerobic exercise glutamine group(DEG).After 6 weeks,the related indicators of glucose and lipid metabolism,anti-oxidative stress and inflammatory factors in diabetic rats were detected,and the possible mechanism affecting inflammatory response were explored.Results:Compared with group N,the levels of serum malondialdehyde(MDA),blood glucose,total cholesterol(TC),triglyceride(TG),insulin,leptin and tumor necrosis factor-α(TNF-α)in group D were increased significantly(P<0.01).Compared with group D,serum levels of MDA,blood glucose,TC,TG,insulin,leptin and TNF-αin three intervention groups were decreased significantly,while the levels of SOD,GSH-Px and adiponectin were increased,and the combined effect was more obvious(P<0.01).Conclusion:Both aerobic exercise and Gln can relieve the glucose and lipid metabolism and disturbance,oxidative stress injury and inflammation in diabetic rats.
作者
王小梅
景会锋
WANG Xiao-mei;JING Hui-feng(Institute of Physical Education,Yanan University,Yanan 716000,China)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2019年第2期150-154,共5页
Chinese Journal of Applied Physiology
基金
陕西省教育厅专项科学研究计划项目(18JK0878)
2018年延安大学科研计划项目(YDQ2018-31)