黑曲霉H9-30全细胞催化合成低聚异麦芽糖

Synthesis of isomaltooligosaccharides by whole-cell Aspergillus niger H9-30

以黑曲霉H9-30全细胞为催化剂转化麦芽糖生产低聚异麦芽糖,通过单因素实验确定其摇瓶最佳转化条件为:温度48 ℃,初始pH值4.2,麦芽糖质量浓度为600 g/L,黑曲霉细胞添加量为15 g/L。此条件下,有效三糖(异麦芽糖、潘糖和异麦芽三糖)占总糖质量分数的50.5%,总低聚异麦芽糖含量为63.3%,达到低聚异麦芽糖工业生产标准。研究结果表明,利用黑曲霉全细胞催化生产低聚异麦芽糖具有较好的操作稳定性,生产IMO-50的半衰期达到20批次(10 d),有望应用于工业化生产低聚异麦芽糖。

Isomaltooligosaccharides (IMOs) are a kind of functional oligosaccharides that have been widely used in food industries. A new procedure for IMOs preparation was developed by using whole-cell of Aspergillus niger H9-30 as a catalyst and maltose as substrate. The optimal reaction condition in shaking flasks was as follows: at 48 ℃ with an initial pH of 4.2, with 600 g/L maltose and 15 g/L A. niger H9-30. Under this condition, the yields of isomaltose, panose, and isomaltotriose accounted for 50.5% of total sugar, and total IMOs content accounted for 63.3%. It was also found that using whole-cell of A. niger to produce IMOs had good operational stability with the half-life reached 20 batches (10 d). In conclusion, A. niger H9-30 has potential use for industrial production of IMO.