摘要
为深入研究小麦祖先种乌拉尔图小麦、粗山羊草的遗传基础、分子结构、进化关系,试验将水稻基因组作为参考基因组进行Blast全基因组比较分析,筛选出8259条长度4kb以上的水稻同源核苷酸序列用circos软件和mauve软件作图。结果表明,小麦祖先种和水稻中存在大量同源核苷酸序列的同线性和共线性排列;祖先种与水稻基因共线性分析表明,3个物种间存在染色体上的共线性,共线性比例达80%以上,并用基因共线性关系对小麦祖先种染色体进化进行了阐述。
To further study the genetic basis,molecular structure and evolutionary relationship of wheat ancestors including Urartu wheat and Aegilops,the rice genome was used as a reference genome for Blast genome-wide comparative analysis.Screening of 8 259 rice homologous nucleotide sequences of 4 kb or longer in length was mapped by circos software and mauve software.The results showed that there were a lot of homologous and collinear alignment of a large number of homologous nucleotide sequences in wheat ancestors and rice.Linear analysis showed that the three species had collinearity on the chromosome,and the collinearity ratio was over 80%.The phylogenetic evolution of wheat ancestors was described by collinearity of genes.
作者
许陶瑜
王长彪
韩斌
赵兴华
刘江
任永康
牛瑜琦
唐朝晖
XU Taoyu;WANG Changbiao;HAN Bin;ZHAO Xinghua;LIU Jiang;REN Yongkang;NIU Yuqi;TANG Zhaohui(College of Bioengineering,Shanxi University,Taiyuan 030006,China;Institute of the Economic Crops,Shanxi Academyof Agricultural Sciences,Fenyang 032200,China;Research Center of Biotechnology,Shanxi Academy of AgriculturalSciences,Taiyuan 030031,China;Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China)
出处
《山西农业科学》
2019年第6期927-930,956,共5页
Journal of Shanxi Agricultural Sciences
基金
山西省重点研发计划项目(201603D221025-2)
山西省农业科学院种业发展专项(2016ZYZX50)
山西省农业科学院重点攻关项目(YGG1624)
关键词
小麦祖先种
水稻
核苷酸序列比对
基因比对
染色体进化
wheat ancestors
rice
nucleotide sequence alignment
gene alignment
chromosome evolution