鲜食枣的快速繁殖体系研究初报

Preliminary Study on Rapid Propagation System of Fresh Jujube

为了建立鲜食枣的快速繁殖体系,以稷山板枣、上海白蒲枣、疙瘩枣、孔府酥脆枣、宁阳六月鲜和尖枣6个鲜食枣品种为材料,研究了适合鲜食枣组织培养的生长调节剂浓度,以及不同的取材部位组织培养的效果。结果表明,启动培养基中生长调节剂浓度6-BA为1.0mg/L,IBA为0.2mg/L时,启动率最高,为63.33%;增殖培养基中生长调节剂浓度6-BA为2.0mg/L,IBA为0.3mg/L时,芽苗粗壮,长势良好,增殖系数最高,为2.33;不同取材部位,启动率表现不同,枣头接种后存活率最高,为53.33%~83.33%,是枣树组织培养快速繁殖很好的启动材料。初步筛选出鲜食枣的快速繁殖体系最适启动培养基为MS+6-BA1.0mg/L+IBA0.2mg/L;增殖培养基为MS+6-BA2.0mg/L+IBA0.3mg/L,最佳外植体材料为枣头。

To establish a rapid propagation system of fresh jujube,six cultivars including Jishanbanzao,Shanghaibaipuzao,Gedazao,Kongfusucuizao,Ningyangliuyuexian and Jianzao were used as materials to study the suitable concentration of growth regulator for tissue culture,and the effect of tissue culture in different parts.The results showed that when the initial medium supplemented with 6-BA 1.0 mg/L and IBA 0.2 mg/L,the rate of initiation was up to 63.33%.When the concentration of 6-BA was 2.0 mg/L and IBA was 0.3 mg/L in the multiplication medium,the multiplication coefficient was up to 2.33,the buds were strong and grew well.The rate of start-up was different in different parts.The survival rate of jujube head after inoculation was the highest (53.33%-83.33%).It was a good starting material for rapid propagation of jujube tissue culture.The best explant material is vegetative shoot of jujube for the rapid propagation system of fresh jujube,the optimal initial medium is MS+6-BA 1.0 mg/L+IBA 0.2 mg/L,the multiplication medium is MS+6-BA 2.0 mg/L+IBA 0.3 mg/L.