高效液相色谱法测定可溶微针贴片中维生素C的含量

Determination of vitamin C in dissolving microneedles by high performance liquid chromatography

目的建立高效液相色谱法测定可溶微针贴片中维生素C的含量。方法采用高效液相色谱法,选择Luna C18100A色谱柱(250 mm×4. 6 mm,5μm);流动相为乙腈-水-冰醋酸(10∶89∶1);流速为0. 6 ml/min;柱温为30℃;检测波长为254 nm。考察方法的专属性、线性、精密度、加样回收率、重复性及稳定性。结果维生素C浓度在2. 32-116μg/ml内与HPLC峰面积呈良好的线性关系(Y=88 754X-188 466,R2=0. 999 0),日内精密度的平均RSD值为0. 74%,日间精密度的平均RSD值为0. 56%,平均加样回收率为105. 86%,方法的专属性和重复性良好,供试品溶液24 h内较稳定。结论本研究所建立的高效液相色谱法测定可溶微针贴片中维生素C的含量简便、准确。

Objective To establish a HPLC method for determining the content of vitamin C in dissolving microneedles array. Methods High performance liquid chromatography was used by Luna C18100 A column( 250 mm × 4. 6 mm,5 μm). Acetonitrile-water-glacial acetic acid( 10 ∶ 89 ∶ 1,V ∶ V ∶ V) was used as the mobile phase. The flow rate was 0. 6 ml/min. The column temperature was 30 ℃ and the detection wavelength was 254 nm. The specificity,linearity,precision,recovery,repeatability and stability of the method were investigated. Results The calibration curve of vitamin C showed good linear relation in the range of 2. 32-116 μg/ml( Y = 88 754 X-188 466,R2= 0. 999 0). The average RSD of intraday precision was 0. 74%,and the average RSD of interday precision was 0. 56%. The average recovery was 105. 86%. The method showed good specificity and repeatability,and the solution was stable in 24 h. Conclusion The established HPLC method for determining the content of vitamin C in dissolving microneedles array is simple and accurate.