青果多糖组分对人宫颈癌Hela细胞增殖的抑制作用研究

Inhibition Effect of Canarii Fructus Polysaccharides on Proliferation of Human Cervical Cancer Hela Cells

目的探讨青果多糖不同组分对人宫颈癌Hela细胞增殖的抑制作用。方法以乙醇除脂、水提、醇沉法制备青果粗多糖,经DEAE-琼脂糖凝胶FF离子交换色谱分离,得CFW(蒸馏水溶液洗脱部位)、CF1(0. 5 mol/L氯化钠溶液洗脱部位)、CF2(1. 0 mol/L氯化钠溶液洗脱部位)和CF3(2. 0 mol/L氯化钠溶液洗脱部位),测定总多糖和总蛋白含量;将不同浓度的上述组分作用于人宫颈癌Hela细胞,以细胞培养液作空白对照,采用四氮唑盐(MTT)法检测Hela细胞增殖程度。结果 4组分总糖含量均大于70%,总蛋白含量均大于0. 87%;与空白对照比较,62. 5,125,250,500,1 000μg/m L CFW,500,1 000μg/m L CF1,1 000μg/m L CF2和CF3的吸光度值显著降低(P <0. 05或P <0. 01)。结论青果CFW组分可显著抑制人宫颈癌Hela细胞的增殖。

Objective To investigate the inhibition effect of different polysaccharides from Canarii Fructus on the proliferation of human cervical cancer Hela cells. Methods Raw polysaccharides was extracted from Canarii Fructus through alcohol degreasing,water extraction,alcohol precipitation and further purified by DEAE-sepharose fast flow ion exchange chromatography to obtain CFW( the washing part of distilled water solution),CF1( the washing part with 0. 5 mol/L sodium chloride solution),CF2( the washing part with 1. 0 mol/L sodium chloride solution) and CF3( the washing part with 2. 0 mol/L sodium chloride solution),the contents of total polysaccharides and proteins were determined. Human cervical cancer Hela cells were treated with the above components at different concentrations,the proliferation of Hela cells was detected by MTT assay with cell culture fluid as blank control. Results The content of total polysaccharide of the 4 components was more than 70%,and the content of total protein of the 4 components was more than 0. 87%.Compared with the blank control,the absorbance values of 62. 5,125,250,500 and 1 000 μg/m L CFW,500 and 1 000 μg/m L CF1,1 000 μg/m L CF2 and CF3 decreased significantly( P < 0. 05 or P < 0. 01). Conclusion The CFW fraction from Canarii Fructus can significantly inhibit the proliferation of human cervical cancer Hela cells.