益母草碱对肉仔鸡生长性能、免疫功能和肠道菌群的影响

Effect of Leonurine on Growth Performance,Intestinal Flora and Immune Function in Broilers

试验旨在研究益母草碱对肉仔鸡生长性能和免疫功能的影响。选取320羽健康、体重相近的1日龄(罗斯308)肉仔鸡,随机分为4组,每组8个重复,分别在基础日粮的基础上添加0、15、30和60mg/kg的益母草碱。试验期为42d。每组分别于21、42日龄测定各组生产性能、免疫器官指标和免疫球蛋白指标。结果表明:(1)各益母草碱组对肉仔鸡全期日采食量、日增重、耗料增重比的影响均不显著。(2)在21、42日龄时各益母草碱组对肉仔鸡胸腺指数、脾脏指数、法氏囊指数无显著影响。(3)60mg/kg益母草碱组对肉仔鸡全期血清中IgA、IgM水平的影响显著,但对血清中IgG含量无显著影响。(4)各益母草碱组肉仔鸡肠道中的大肠杆菌、沙门氏菌有显著抑制作用,对乳酸杆菌无显著影响。研究结果提示,日粮中添加益母草碱对肉仔鸡生长性能、免疫器官指数无显著影响,但能提高血清中免疫球蛋白的含量,抑制肠道内的大肠杆菌和沙门氏菌数量,保护乳酸杆菌数量,调节肠道菌群平衡。

To investigate the effect of Leonurine on growth performance and immune function of broilers,a total of 320 healthy broilers with similar body weight at 1-day-old(Ross 308)were randomly allotted to 4 treatment groups consisting of 8 replicates,adding 0,15,30 and 60mg/kg Leonurine in the basic diet respectively.The feeding trial lasted for 42 days.The production performance,immune organ index and immunoglobulin index were measured at 21 and 42 days of age.The results showed that 1)Leonurine had no significant effects on daily feed intake,daily gain and feed-to-weight ratio of broilers in each group.2)Leonurine had no significant effect on thymus index,spleen index and bursal index.3)The level of serum Ig A,Ig M in broilers was significantly increased in the 60 mg/kg dose group,but there was no significant effect on serum Ig G.4)Leonurine had a significant inhibitory effect on E.coli and Salmonella in the intestine of broilers,but had no significant effect on Lactobacillus.Consequently,dietary supplementation of Leonurine had no significant effect on growth performance and immune organ index of broilers,but it could increase the content of immunoglobulin in the serum,inhibit the number of E.coli and Salmonella in the intestine,protect the amount of Lactobacillus,and regulate the intestinal flora balance.