不同FSH注射策略对澳洲白绵羊超数排卵效果的分析

Effects of Different FSH Injection Strategies on Superovulation of Australian White Sheep

绵羊超数排卵过程中,供体的卵泡刺激素(FSH)注射策略会对超数排卵的效果产生显著影响。本研究以澳洲白绵羊为供体,采用随机分组法将555只供体羊随机分为组1、组2和组3。在饲养环境和FSH、孕马血清促性腺激素(PMSG)注射总剂量相同情况下,供体母羊情期11~14d采用FSH+PMSG注射法,按照不同注射策略设定方案1、方案2和方案3。结果表明:方案1平均获得胚胎数(5.49±2.78)枚,可用胚胎数为(4.76±3.12)枚;方案2平均获得胚胎数(4.22±3.04)枚,平均可用胚胎数为(2.87±3.12)枚,方案3平均获得胚胎数(7.79±3.78)枚,平均可用胚胎数为(6.96±4.40)枚。3种方案的超数排卵效果差异极显著(P<0.01),方案1优于方案2(P<0.01),方案3优于方案1和方案2(P<0.01),研究结果提示,澳洲白绵羊超数排卵的效果与FSH注射策略相关。

In the process of superovulation in sheep,the hormone treatment of the donor has a significant effect on the effect of superovulation.In this study,Australian White sheep were used as donors,and 555 donor sheep were randomly divided into group 1,group 2 and group 3 using a random grouping method.With the same total dose of FSH and PMSG injections and the same rearing environment,the FSH+PMSG injection method was applied to ewes at estrogen period from 11 to 14 days,and scheme 1,scheme 2 and scheme 3 were set according to different FSH injection strategies.The results showed that the average number of embryos obtained in scheme 1 was 5.49±2.78,and the number of available embryos was 4.76±3.12.The average number of embryos obtained in scheme 2 was 4.22±3.04,and the average number of available embryos was 2.87±3.12.The average number of embryos obtained in scheme 3 was 7.79±3.78,with the average number of available embryos being 6.96±4.40.Three kinds of schemes of superovulation effect was extremely different(P<0.01),scheme 1 was better than 2(P<0.01),scheme 3 was better than that of scheme 1 and 2(P<0.01).It could be seen that the effect of superovulation in Australian white sheep was related to the FSH injection strategy.