盘状大同源物5在肺腺癌中的表达及其临床意义

Expression of DLG5 in Lung Adenocarcinoma and its Clinical Significance

目的:探讨盘状大同源物5(discs large homolog 5,DLG5)在肺腺癌中的表达及其临床意义。方法:采用免疫组化SP法检测122例Ⅰ期和ⅢA期肺腺癌组织中DLG5的表达情况,并采用Western blotting法检测20对肺腺癌组织癌组织和癌旁组织DLG5的表达情况。统计患者的临床资料,分析DLG5表达与临床特征的相关性,采用Kaplan-Meier曲线对随访数据进行预后分析。结果:Ⅰ期肺腺癌组织中DLG5的阳性表达率明显高于ⅢA期肺腺癌组织(P<0.01)。肺腺癌组织中DLG5的阳性表达率明显低于癌旁肺组织。DLG5表达阳性的患者,5年生存率(OS)明显高于表达阴性的患者(51.1%vs 28.1%,P<0.01)。肺腺癌组织中DLG5的阳性表达水平与淋巴结转移(P<0.01)、TNM分期(P<0.01)和肿瘤复发(P<0.01)呈负相关。生存分析显示DLG5和TNM分期是肺腺癌的独立预后影响因素。结论:DLG5在肺腺癌中表达下降,DLG5可能对肺腺癌患者的预后有一定的指导意义。

Objective To investigate the expression and clinical significance of discs large homolog 5(DLG5)in lung adenocarcinoma(AC).Methods SP immunohistochemistry was used to detect the expression of DLG5 in 122 cases of lung AC tissues.Western blotting was used to detect the expression of DLG5 in 20 pairs of lung AC tissues and adjacent tissues.The clinical data were detected to analyze the relationship between the expression and clinical characteristics.Kaplan-Meier curves were used to conduct survival analysis of follow-up data.Results The positive expression rate of DLG5 in stageⅠlung AC was significantly higher than that in stageⅢA(P<0.01).The positive expression rate of DLG5 in lung AC tissues was significantly lower than that in adjacent tissues.In patients with positive DLG5 expression,the 5-year survival rate(OS)was significantly higher than that in patients with negative DLG5 expression(51.1%vs 28.1%,P<0.01).The positive expression of DLG5 in AC was negatively correlated with lymph node metastasis(P<0.01),TNM stage(P<0.01)and recurrence(P<0.01).Survival analysis showed that DLG5 and TNM stage were independent prognostic factors for lung AC.Conclusion The expression of DLG5 is decreased in lung AC,and maybe helpful for the prognosis of patients with lung AC.