响应面法优化小球藻超声波破壁技术

Optimization of Ultrasound Technology for Chlorella sp. Cell-breakage Using Response Surface Methodology

小球藻营养丰富且全面,具有重要的生理功能和保健功效,在营养品、保健品、功能食品方面具有良好的应用前景。然而,由于小球藻的细胞壁十分坚硬,破碎困难,胞内的有效成分不易释放,抑制了其产业化发展。因此,一种安全有效的破壁方法是小球藻产业化发展的关键。该研究采用超声波破壁技术处理小球藻,通过单因素试验考察超声时间、超声波功率、料液比对细胞破碎的影响,并进一步采用Box-Behnken试验设计和响应面分析法优化其工艺参数。结果表明:通过单因素试验得到破壁效果的影响力大小依次为超声时间、超声波功率、料液比。Design-Expert8.0.6软件优化的工艺条件为料液比1∶200(g∶mL)、超声波功率为450W、超声时间为15min。该条件下细胞破壁率为79.51%,与模型预测值80%非常接近。采用响应面法对超声波破碎藻细胞条件进行优化合理可行。

Chlorella has been applied in many fields,for its rich,all-round and balanced nutrition.The cell wall of Chlorella is very hard to crush,so that the activate component in the cell is difficult to release.Then,industrialization development for Chlorella is restricted.Therefore,a safety and effective method to break cell is the key technology in Chlorella industry.Ultrasound technology was adopted to break Chlorella cell in this study.In order to obtain the optimum parameters of ultrasound technology,the effects of several factors including solid-liquid ratio,ultrasonic power and process time on the breaking were investigated by using a single factor experiment and further optimized by using Box-Behnken experiment and response surface methodology (RSM).The results showed that process time had the most important influence on cell-breakage and it was followed by ultrasonic power ,solid-liquid radio in the effects.The optimal condition of cell-breakage was obtained,that was,solid-to-liquid ratio of 1 ∶200,ultrasonic power of 450 W and process time of 15 min.The cell breaking rate was 79.51% under those conditions,which perfectly matched the predicted value (80%).Therefore,it is feasible that utilizing RSM to optimize the conditions of cell-breakage.