不同乳酸菌发酵乳对高血脂症大鼠肠道菌群的影响

Influence of Different Lactic Acid Bacteria Fermented Milk on Gut Microbiota of Hyperlipidemia Rats

试验旨在探究不同乳酸菌发酵乳对高血脂模型大鼠血脂与肠道菌群的调节作用。将体重为(120±10)g的 40 只 SD 雄性大鼠随机分为空白对照组(C 组)、高脂模型组(M 组)、L. rhamnosus LV108 发酵乳组(LV组)及混合乳酸菌(LV108 发酵乳∶grx08 发酵乳∶grx12 发酵乳=2∶1∶1)发酵乳组(H 组),每组 10 只,建模后干预(1 mL/100 g,1×108 CFU/mL)4 周,测定大鼠血脂及大鼠粪样细菌 16S rDNA 的 V3-V4 区,并对大鼠肠道微生物和血清血脂水平的相关性进行分析。结果表明:LV 组和 H 组大鼠血清中甘油三酯、总胆固醇及低密度脂蛋白胆固醇含量显著降低,体重增长被抑制;LV 组和 H 组中大鼠肠道菌群的丰度及多样性显著提高,且 H 组大鼠肠道菌群的丰度高于 LV 组(P<0.05);LV 组和 H 组大鼠粪便中 Lactobacillus spp.、S24-7 spp 和 Treponema spp.等菌属显著升高,Peptostreptococcus spp.、Clostridium spp.、Turicibacter spp、Escherichia spp.及 Desulfovibrio spp.等菌属显著降低。结果提示,L. rhamnosus LV108发酵乳及混合乳酸菌发酵乳均能改善大鼠血脂水平并恢复大鼠肠道菌群的多样性及菌群构成。

To explore the effects of different lactic acid bacteria fermented milk on the regulation of blood lipids and intestinal flora in hyperlipidemic model rats, Totally 40 SD male rats were randomly divided into 4 groups (C, M, LV and H). After a 8-wk experimental period, The level of blood lipid was tested and the fecal bacterial composition was investigated of the V3- V4 region of the 16S rRNA gene, then the rat intestinal microbes and the correlation of serum lipid levels were analyzed. The results showed that group LV108 and group H could signi ficantly reduce triglyceride, total cholesterol, low density lipoprotein cholesterol and inhibit weight gain.. Group LV108 and group H signi ficantly increased the abundance and diversity of rat intestinal flora (P<0.05). The abundance of the intestinal flora of group H was significantly higher than that of the group LV(P<0.05). At the same time, both groups promoted the growth of Lactobacillus spp., S24-7 spp., and Treponema spp. and other genus. The proliferation of microorganisms such as Streptococcus spp., Clostridium spp., Turicibacter spp., Escherichia spp., and Desulfovibrio spp. were inhibited (P<0.05). L. rhamnosus LV108 fermented milk and mixed lactic acid bacteria fermented milk can improve the blood lipid level of rats and restore the diversity of the intestinal flora and the composition of the flora.