二苯乙烯苷对AngⅡ诱导人脐静脉内皮细胞衰老的保护作用

Protective effects of 2,3,5,4-tetrahydroxystilbene-2-O-β-D-glucoside on AngⅡ-induced human umbilical vein endothelia cellular senescence

目的探讨二苯乙烯苷对血管紧张素Ⅱ(AngⅡ)诱导人脐静脉内皮细胞衰老的保护作用。方法体外培养人脐静脉内皮细胞后,AngⅡ诱导细胞衰老模型,MTS法检测细胞存活率,SA-β-gal染色分析细胞衰老程度,qPCR检测miR-34a mRNA表达,Western blot检测p53、PAI-1、SIRT1、E2F1蛋白表达。结果二苯乙烯苷干预后,细胞存活率、SIRT1蛋白表达显著升高(P<0.05),β-gal阳性率及p53、PAI-1蛋白表达显著降低(P<0.05)。同时,该成分对miR-34a、E2F1表达也有明显抑制作用(P<0.05)。结论二苯乙烯苷对AngⅡ诱导人脐静脉内皮细胞衰老具有明显保护作用,其作用机制可能与抑制miR-34a、E2F1表达及促进SIRT1蛋白表达有关。

AIM To explore the protective effects of 2,3,5,4-tetrahydroxystilbene-2-O-β-D-glucoside on AngⅡ-induced human umbilical vein endothelial cellular senescence.METHODS After in vitro cultivation of human umbilical vein endothelial cells, the cellular senescence model was induced by Ang II, MTS assay, SA-β-gal staining, qPCR and Western blot were performed to detect cell viability, analyze cellular senescence degree, and investigate miR-34a mRNA expression, and the protein expressions of p53, PAI-1, SIRT1, E2F1, respectively.RESULTS After 2,3,5,4-tetrahydroxystilbene-2-O-β-D-glucoside intervention, significantly increased cell viability and SIRT1 protein expression(P<0.05), and markedly decreased β-gal positive rate and p53, PAI-1 protein expressions(P<0.05) were found. Meanwhile, this constituent also demonstrated its obvious inhibition on the expressions of miR-34 a and E2F1(P<0.05).CONCLUSION 2,3,5,4-Tetrahydroxystilbene-2-O-β-D-glucoside exhibits obvious protective effects on AngⅡ-induced human umbilical vein endothelial cellular senescence, whose mechanisms may contribute to its inhibition on miR-34 a, E2F1 expressions and promotion on SIRT1 protein expression.