HPLC法同时测定淫羊藿-骨碎补药对中7种成分

Simultaneous determination of seven constituents in herbal pair of Epimedii Folium and Drynaria Rhizoma by HPLC

目的建立HPLC法同时测定淫羊藿-骨碎补药对中朝藿定A、新北美圣草苷、柚皮苷、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅰ的含有量。方法淫羊藿-骨碎补饮片60%乙醇提取物的分析采用采用Waters X Bridge C18柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;柱温25℃;体积流量1.0 mL/min;检测波长280 nm。结果 7种成分在各自范围内线性关系良好(r≥0.9991);平均加样回收率95.06%~103.9%,RSD 1.1%~2.7%。结论该方法准确稳定,重复性好,可用于淫羊藿-骨碎补药对的质量控制。

AIM To establish an HPLC method for the simultaneous content determination of epimedin A, neoeriocitrin, naringin, epimedin B, epimedin C, icariin and baohuoside Ⅰ in herbal pair of Epimedii Folium-Drynaria Rhizoma.METHODS The analysis of 60% ethanol extract of Epimedii Folium-Drynaria Rhizoma was performed on a 25 ℃ thermostatic Waters X Bridge C18 column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS Seven constituents showed good linear relationships within their own ranges(r ≥ 0.9991),whose average recoveries were 95.06%-103.9% with the RSDs of 1.1%-2.7%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of herbal pair,Epimedii Folium-Drynaria Rhizoma.