乳果糖增加正常小鼠肠道益生菌群的种类和数量

Lactulose increases species and quantity of intestinal microbiota in normal mice

目的基于16SrDNA菌群测序技术,研究乳果糖、益生菌联合乳果糖对正常小鼠肠道菌群的影响。方法将C57BL/6J小鼠分为对照组、乳果糖组和益生菌联合乳果糖组(益生菌为双歧杆菌和乳杆菌的混合制剂)。收集灌胃前、灌胃1周后和灌胃2周后的粪便标本和处死后的黏膜标本送检菌群测序。结果与对照组相比,乳果糖组的乳杆菌属(0.1131/0.0515,P<0.01)和Akkermansia增加(0.0391/0.0011,P<0.001);益生菌联合乳果糖组乳杆菌属(0.1041/0.0407,P<0.05)和Faecalibacterium增加(0.0295/0.0070,P<0.05),而脱硫弧菌属减少(0.0052/0.0149,P<0.05)。结论乳果糖和益生菌联合乳果糖可以增加有益菌属,减少有害菌属,对肠道菌群产生调节作用。

Objective To investigate the effects of lactulose or probiotics combined with lactulose on gut flora of normal mice based on 16 S rDNA sequencing technology. Methods C57 BL/6 J mice were divided into 3 groups(A, B and C). Group A was blank control group, group B and group C were given lactulose or probiotics combined with lactulose for 2 weeks respectively. Probiotics were mixture of Bifidobacterium and Lactobacillus. Fecal samples collected before gavage, 1 week after gavage, and 2 weeks after gavage and mucosal specimens collected from executed animal, were sequenced for 16 S rDNA. Results Compared with blank control group, Lactobacillus and Akkermansia were increased(0.113 1/0.051 5, P<0.01;0.039 1/0.001 1, P<0.001) in lactulose group. Lactobacillus(0.104 1/0.040 7, P<0.05) and Faecalibacterium(0.029 5/0.007 0, P<0.05) counting were higher and the counting of Desulfovibrio(0.005 2/0.014 9, P<0.05) was lower in probiotics combined with lactulose group compared with blank control group. Conclusions Lactulose or probiotics combined with lactulose can increase beneficial bacteria and reduce pathogenic bacteria so to regulate the intestinal microbiota.