摘要
使用不同浓度的α-酮戊二酸处理金针菇(Flammulina filiformis)Dan3菌丝,结果显示低浓度α-酮戊二酸(1~5mmol/L)促进菌丝生长,高浓度α-酮戊二酸(10~20mmol/L)抑制其生长。金针菇赖氨酸合成途径中有3个基因(HCS、HIDH以及SDH)的表达模式呈α-酮戊二酸低浓度促进表达,而高浓度抑制表达;另有4个基因(HAH、AAR、AAT2和SDR)在添加α-酮戊二酸后表达量下降。金针菇菌丝中赖氨酸含量也显示出被低浓度α-酮戊二酸促进而被高浓度抑制的现象,2.5mmol/L是诱导赖氨酸合成的最佳浓度。
The mycelia of F. filiformis Dan3 were treated with different concentrations of α-ketoglutaric acid. The results showed that the mycelia growth was accelerated by low concentrations of α-ketoglutaric acid (1 ~ 5 mmol/L) and inhibited by high concentrations (10 ~ 20 mmol/L). Further study showed that the expression levels of three genes (HCS, HIDH and SDH) in the lysine synthesis pathway of F. filiformis increased when the concentration of α-ketoglutaric acid was low, but decreased when the concentration of α-ketoglutaric acid was high. The expression levels of four other genes (HAH, AAR, AAT2 and SDR) decreased after addingα-ketoglutaric acid. The lysine content in F.filiformis showed the same trend as increased at low concentration of α-ketoglutaric acid and reduced at high concentration.2.5 mmol/L was the best concentration to induce lysine synthesis.
作者
刘建雨
蒋佩钰
王瑞娟
徐珍
张丹
宋春艳
于海龙
章炉军
张美彦
谭琦
李巧珍
尚晓冬
LIU Jianyu;JIANG Peiyu;WANG Ruijuan;XU Zhen;ZHANG Dan;SONG Chunyan;YU Hailong;ZHANG Lujun;ZHANG Meiyan;TAN Qi;LI Qiaozhen;SHANG Xiaodong(Institute of Edible Fungi , Shanghai Academy of Agricultural Sciences , Key Laboratory of Edible Fungi Resources and Utilization (South ), Ministry of Agriculture , P. R. China , National Engineering Research Center of Edible Fungi , Shanghai Key Labotatory of Agricultural Genetics and Breeding , Shanghai 201403 , China)
出处
《食用菌学报》
CSCD
北大核心
2019年第2期10-16,共7页
Acta Edulis Fungi
基金
上海市市级农口系统青年人才成长计划项目[沪农青字(2017)第1-13号]
上海市农业科学院卓越团队建设计划[2017(A-02)]
上海市食用菌产业技术体系建设专项资金[沪农科产字(2019)第9号]
关键词
金针菇
Α-酮戊二酸
赖氨酸
基因表达
Flammulina filiformis
α-ketoglutaric acid
lysine
gene expression
