水鬼蕉生物碱对人肝癌HepG-2细胞凋亡的影响

Effects of Alkaloids of Hymenocallis littoralis on Apoptosis of Human Hepatocellular Carcinoma HepG-2 Cells

为揭示水鬼蕉生物碱(AHL)对HepG-2细胞作用,采用FITC-Annexin V/PI双染色法检测AHL对HepG-2细胞凋亡的影响,并检测了AHL对HepG-2细胞内Ca2+浓度、活性氧(ROS)、pH的影响。结果表明:AHL可以通过细胞内ROS、Ca^2+、pH三者间相互作用而诱导细胞凋亡。

In order to reveal the effect of alkaloids of Amaryllidaceae plant Hymenocallis littoralis (AHL) on HepG-2 cells, FITC-Annexin V/PI double staining was used to detect the effect of AHL on apoptosis of HepG-2 cells and on Ca^2+ concentration, reactive oxygen species (ROS) and pH in HepG-2 cells. The results show that: AHL could induce apoptosis through the interaction of ROS, Ca^2+, and pH in cells.