miR-34a和XIAP在不同基因分型乳腺癌中的表达及意义

Expression and significance of miR-34a and X-linked inhibitor of apoptosis protein in different genotypes of breast cancer

目的检测不同基因分型乳腺癌患者中miR-34a和X染色体连锁凋亡抑制蛋白(XIAP)表达水平并探讨其临床意义。方法选取2015年11月至2017年12月诊治并明确基因分型的乳腺浸润性导管癌患者125例,其中管腔A型、管腔B型、管腔B-like型、ERBB2+型和基底样型各25例。采用实时荧光定量PCR(qRTPCR)技术检测不同基因分型乳腺癌患者肿瘤组织中miR-34a和XIAP mRNA水平,采用二步免疫组化法检测XIAP蛋白水平。结果管腔A型组、管腔B型组、管腔B-like型组、ERBB2+型组、基底样型组患者肿瘤组织miR-34a及XIAP表达水平比较,差异均有统计学意义(P<0.05)。miR-34a表达与患者肿瘤组织学分级显著相关(P<0.05);与年龄、是否绝经、肿瘤大小、肿瘤距乳头距离、淋巴结转移情况、人表皮生长因子受体-2(HER2)状态、激素受体(ER/PR)状态均无显著相关(P>0.05)。XIAP表达与患者肿瘤组织学分级显著相关(P<0.05);与年龄、是否绝经、肿瘤大小、肿瘤距乳头距离、淋巴结转移情况、HER2状态、ER/PR状态均无显著相关(P>0.05)。miR-34a和XIAP在各基因分型乳腺癌中的表达呈负相关(r=-0.602,P<0.05)。结论 miR-34a和XIAP在不同基因分型乳腺癌组织中存在差异性表达,提示联合检测miR-34a和XIAP对不同基因分型乳腺癌具有一定预测价值。

Objective To detect the expression levels of miR-34 a and X-linked inhibitor of apoptosis protein(XIAP) in patients with different genotypes of breast cancer and to explore its clinical significance.Methods A total of 125 patients with breast invasive ductal carcinoma(BIDC) who were diagnosed and genotyped in our hospital from November 2015 to December 2017 were selected, including luminal type A(n=25), luminal type B(n=25), luminal B-like type(n=25), ERBB2+ type(n=25) and basal-like type(n=25). Real-time quantitative PCR(qRTPCR) was used to detect the mRNA levels of miR-34 a and XIAP in tumor tissues of patients with different genotypes of breast cancer. The level of XIAP protein was detected by two-step immunohistochemistry. Results There were significant differences in the expression levels of miR-34 a and XIAP among luminal type A group, luminal type B group, luminal B-like group, ERBB2+ group and basal-like group(P<0.05). The expression of miR-34 a was significantly correlated with the histological grade of the tumor(P<0.05), however,which was not significantly correlated with age, menopause, tumor size, distance from the tumor to the nipple, lymph node metastasis, human epidermal growth factor receptor-2(HER2) status and hormone receptor(ER/PR) status(P>0.05). XIAP expression was significantly correlated with histological grade of the tumor(P<0.05), which was, however, not correlated with age, menopause, tumor size, distance from the tumor to the nipple, lymph node metastasis, HER2 status, and ER/PR status(P>0.05). There was a negative correlation between miR-34 a and XIAP expression in various genotypes of breast cancer(r=-0.602,P<0.05). Conclusion The miR-34 a and XIAP are differentially expressed in different genotypes of breast cancer, which suggests that combined detection of miR-34 a and XIAP has a certain predictive value for different genotypes of breast cancer.