摘要
沉积物样品用乙腈-柠檬酸缓冲溶液提取后经HLB-SPE柱净化,采用LC-MS/MS法在MRM模式下测定17种磺胺类和4种四环素类抗生素,通过优化测定条件,使方法在0.500μg/kg^100μg/kg范围内线性良好。方法检出限为0.15μg/kg^1.2μg/kg,2个质量比水平的加标回收率为44.1%~125%,试验5次结果的RSD<16.0%。将该方法用于测定厦门近岸海域沉积物中抗生素,共有6种磺胺类和4种四环素类抗生素检出,测定值为0.3μg/kg^26.2μg/kg。
Sediment samples were extracted by acetonitrile citric acid buffer solution and purified by HLB-SPE column. Seventeen sulfonamides and four tetracyclines were determined by liquid chromatography-tandem mass spectrometry under multi-reaction monitoring mode(MRM). By optimizing the determination conditions, the linearity of the method was good in the range of 0.500 μg/kg^100 μg/kg. The detection limits were 0.15 μg/kg ~1.2 μg/kg. The recoveries of standard addition were in range of 44.1%~125% at two mass ratio levels. The RSD of 5 duplicate measurements was less than 16.0%. The method was applied to determine antibiotics in sediments from Xiamen coastal area in Fujian Province. Six sulfonamides and four tetracyclines were detected and the content ranged in 0.3 μg/kg^26.2 μg/kg.
作者
陈秋兰
CHEN Qiu-lan(Quanzhou Environmental Monitoring Center Station, Quanzhou, Fujian 362000, China)
出处
《环境监测管理与技术》
CSCD
2019年第3期49-52,共4页
The Administration and Technique of Environmental Monitoring
基金
福建省环保科技计划基金资助项目(2016R004)
