胸腔积液凝集物在组织脱落细胞学诊断中的应用

Application of Pleural Coagulants in Cytological Diagnosis of Tissue Exfoliation

目的探讨胸腔积液凝集物石蜡包埋切片在细胞学诊断的意义。方法对105例住院患者产生凝集物的胸腔积液进行拉片和凝集物包埋连续切片,HE染色及免疫组织化学染色。结果105例临床送检有凝集物的胸腔积液标本,采用细胞涂片方法检出恶性肿瘤84例,5例判断不清,16例非肿瘤性间皮增生。采用细胞蜡块方法检出恶性肿瘤88例,17例非肿瘤性间皮增生。采用胸腔积液凝集物方法检出恶性肿瘤88例,17例非肿瘤性间皮增生。结论胸腔积液凝集物石蜡包埋切片避免了细胞涂片的局限性,切片细胞丰富,易于观察组织脱落细胞的组织学形态,可应用免疫组织化学提高脱落细胞学诊断价值,可降低脱落细胞学的漏诊和误诊风险。

Objective Investigate the significance of paraffin embeded pleural agglutination section in cytological diagnosis.Methods Totally 105 inpatient cases were collected.Cell smear method,cell block method and pleural agglutination method were used to obtain slides of cells in pleural.HE staining and immunohistochemical staining(IHC) were conducted on the slides.Results Eighty four cases of cancer,16 cases of non-tumor mesotheliosis,5 cases of unclear diagnosis were detected with cell smear method.88 cases of cancer,17 cases of non-tumor mesotheliosis were detected with cell block method.88 cases of cancer,17 cases of non-tumor mesotheliosis were detected with pleural agglutination method.Conclusion The paraffin embeded section of the pleural water agglutinate avoids the limitation of the cell smearmethod,and is easy to observe the histological morphology of tissue exfoliation cells.It can be used to improve the diagnostic value of exfoliation cytology by using immunohistochemistry method,and can reduce the risk of missed diagnosis and misdiagnosis of exfoliation cytology.