食管癌组织环状-TTBK2和miR-217表达及临床病理意义

Expression of Circular-TTBK2 and miR-217 in Esophageal Carcinoma and its Clinicopathological Significance

目的检测miR-217、环状-TTBK2在食管癌肿瘤组织和癌旁组织中表达情况,探讨miR-217、环状-TTBK2与食管癌患者临床病理特征及预后的关系。方法收集食管癌肿瘤组织及对应癌旁组织各69例,实时荧光定量PCR(qRT-PCR)检测miR-217、环状-TTBK2表达水平,比较并分析miR-217、环状-TTBK2与食管癌患者临床病理特征及预后的关系。结果食管癌肿瘤组织中miR-217表达水平显著低于癌旁组织,差异有统计学意义(P<0.05);环状-TTBK2表达水平显著高于癌旁组织(P<0.05)。食管癌肿瘤组织中miR-217与环状-TTBK2表达呈负相关(r=-0.609,P<0.05)。miR-217、环状-TTBK2表达水平高低与食管癌患者TNM分期、肿瘤分化程度及淋巴结转移有关(P<0.05),与患者年龄、性别、病变部位、病理类型、浸润深度无关(P>0.05)。miR-217低表达患者3年存活率(41.46%)显著低于miR-217高表达者(78.57%,P<0.05),环状-TTBK2低表达者3年存活率(37.21%)明显低于环状-TTBK2高表达者(88.46%,P<0.05)。COX多因素分析显示,miR-217表达、环状-TTBK2表达、TNM分期是影响食管癌患者预后的独立危险因素(HR=2.674,95%CI:1.128~6.339;HR=2.328,95%CI:1.097~4.940;HR=2.907,95%CI:1.573~5.372;P均<0.05)。结论miR-217在食管癌患者肿瘤组织中低表达,环状-TTBK2高表达,二者表达与食管癌疾病恶性程度及患者预后有关。

Objective To detect the expressions of miR-217 and ring-TTBK2 in tumor tissues and paracancerous tissues of esophageal cancer,and investigate the relationships between miR-217 and ring-TTBK2 with the clinicopathological characteristics and prognosis in patients with esophageal cancer.Methods 69 cases of tumor tissues of esophageal cancer and corresponding paracancerous tissues were collected.Real time fluorescence quantitative PCR (qRT-PCR) was used to detect the expression levels of miR-217 and ring-TTBK2.The relationship between miR-217 and ring-TTBK2 with clinicopathological characteristics and prognosis of esophageal cancer was compared and analyzed.Results The expression level of miR-217 in tumor tissues of esophageal cancer was significantly lower than that in paracancerous tissues,and the difference was statistically significant ( P <0.05).The expression level of ring-TTBK2 was significantly higher than that in paracancerous tissues ( P <0.05).The expression of miR-217 was negatively correlated with the expression of ring-TTBK2 in tumor tissues of esophageal cancer ( r =-0.609,P <0.05).The expression levels of miR-217 and ring-TTBK2 were correlated with TNM stage,differentiation degree and lymph node metastasis of patients with esophageal cancer ( P <0.05).They were not related to age,sex,lesion location,pathological type and depth of invasion ( P >0.05).The 3 year survival rate of patients with low expression of miR-217 (41.46%) was significantly lower than that of patients with high miR-217 expression (78.57%,P <0.05).The 3 year survival rate of patients with low expression of ring-TTBK2 (37.21%) was significantly lower than that of patients with ring-TTBK2 with high expression (88.46%,P <0.05).COX multifactor analysis showed that miR-217 expression,ring-TTBK2 expression and TNM staging were independent risk factors for the prognosis of patients with esophageal cancer (HR 2.674,95% CI 1.128-6.339;HR 2.328,95% CI 1.097-4.940;HR 2.907,95% CI 1.573-5.372;P <0.05).Conclusion MiR-217 is low expressed in tumor tissues of esophageal cancer patients,and the ring-TTBK2 is high expressed.Their expression is associated with malignant degree of esophageal cancer and prognosis of patients.