清热消癜方对新诊断原发免疫性血小板减少症患者淋巴细胞亚群、CD4^+ CD25^+ CD127low^+ Treg细胞的影响

The Influence of Heat-Clearing and Purpura-Removing Prescription on Lymphocyte Subsets and CD4^+ CD25^+ CD127low^+ Treg Cells of Patients with Newly Diagnosed Primary Immune Thrombocytopenia

目的:观察清热消癜方对新诊断原发免疫性血小板减少症(primary immune thrombocytopenia,ITP)患者淋巴细胞亚群、CD4^+ CD25^+ CD127low^+ Treg细胞的影响。方法:选取2014年4月至2017年3月在本院诊治的新诊断ITP患者63例,采用随机数字表法分为观察组32例和对照组31例。对照组给予醋酸泼尼松治疗,观察组在对照组治疗的基础上加用清热消癜方治疗。观察两组患者治疗前后血小板计数(platelet,PLT),流式细胞术检测淋巴细胞亚群、CD4^+ CD25^+ CD127low^+ Treg细胞的百分率。结果:观察组有效率96. 88%,对照组有效率93. 55%,两组有效率比较,差异无统计学意义(P> 0. 05);观察组治疗后PLT水平明显优于对照组,差异有统计学意义(P <0. 01);观察组治疗后淋巴细胞亚群水平优于对照组(P <0. 05或P <0. 01);观察组治疗后CD4^+ CD25^+ CD127low^+ Treg细胞水平优于对照组(P <0. 05)。结论:清热消癜方能显著改善新诊断ITP患者血小板计数水平,可通过调节异常的淋巴细胞亚群、上调CD4^+ CD25^+ CD127low^+ Treg细胞水平和下调CD19^+水平发挥作用。

Objective: To observe the influence of Heat-Clearing and Purpura-Removing Prescription on lymphocyte subsets and CD4^+ CD25^+ CD127 low^+ immunologic function of patients with newly diagnosed primary immune thrombocytopenia( ITP). Methods: Sixty-three newly diagnosed ITP patients treated in our hospital from April 2014 to March 2017 were. They were selected and divided into the observation group with 32 cases and the control group with 31 cases according to random number table. The ones in the control group were treated with prednisone acetate,while the ones in the observation group were treated with Heat-Clearing and Purpura-Removing Prescription on the basis of the control group. The platelet counts( PLT) before and after treatment. were observed;the lymphocyte subsets and percentage of CD4^+ CD25^+ CD127 low^+ Treg cells were measured by flow cytometry. Results: The effective rate of the observation group was 96. 88%,and that of the control group was 93. 55%. There was no significant difference between the two groups( P > 0. 05). The platelet count level of the observation group was significantly better than that of the control group after treatment,and the difference was statistically significant( P < 0. 01). After the treatment,the level of lymphocyte subsets in the observation group was better than that in the control group( P < 0. 05 or P < 0. 01),and the level of CD4^+ CD25^+ CD127 low^+ Treg cells in the observation group was better than that in the control group( P < 0. 05). Conclusion:Heat-Clearing and Purpura-Removing Prescription can significantly improve the platelet count level in newly diagnosed ITP patients by regulating abnormal lymphocyte subsets,up-regulating CD4^+ CD25^+ CD127 low^+ Treg cell level and down-regulating CD19^+ level.