摘要
目的探究长链非编码RNA母系表达基因3(lncRNA MEG3)对头颈鳞癌细胞增殖和侵袭的影响。方法体外培养头颈部鳞癌细胞系HN6转染MEG3,分为MEG3阴性对照组(MEG3对照组)、MEG3慢病毒过表达转染组(MEG3过表达组)。实时荧光定量PCR(qRT-PCR)检测MEG3 mRNA表达;CCK-8、transwell检测细胞增殖、侵袭耐力;蛋白免疫印迹法(western blottingWB)检测细胞中ZEB2、波形蛋白(vimentin)、E-钙黏着蛋白(E-cadherin)蛋白表达情况。结果在HN6细胞系中,与MEG3对照组相比,MEG3过表达组细胞中MEG3 mRNA表达升高(P<0.05)。与阴性对照组相比,MEG3过表达组细胞增殖、侵袭能力均降低(P<0.05)。与阴性对照组相比,MEG3转染组ZEB2、vimentin蛋白表达降低,E-cadherin蛋白表达升高(P<0.05)。结论 MEG3表达升高后可抑制头颈鳞癌细胞的增殖、侵袭,其机制可能与抑制细胞上皮-间充质转化有关。
Objective To investigate the effects of long non-coding RNA maternally expression gene 3(MEG3) on the proliferation and invasion of head and neck squamous cell carcinoma(HNSCC). Methods The head and neck squamous cell carcinomacell line HN6 was cultured in vitro,and they were divided into negative transfection group(MEG3 control) and MEG3 Lentivirus overexpression transfection group(MEG3 overexpression) after the transfection of MEG3. Real-time quantitative PCR(qRT-PCR) was used to detect MEG3 mRNA expression. CCK-8 assay and transwell were used to detect cell proliferation and invasion. Western blotting(WB) was used to detect the expressions of ZEB2, Vimentin and E-cadherin in cells. Results In HN6 cell,MEG3 mRNA expression increased in the MEG3 overexpression group compared with the MEG3 control group(P<0.05). Compared with the control group, cell proliferation and invasion in the MEG3 overexpression group decreased(P<0.05). Compared with control group, the expressions of ZEB2 and vimentin protein in MEG3 overexpression group decreased, while the expression of E-cadherin protein increased(P<0.05). Conclusion The increase of MEG3 expression can inhibit the proliferation and invasion of head and neck squamous cell carcinomacells,whose mechanism may be related to the inhibition of epithelial cell mesenchymal transition(EMT).
作者
季叶枫
于洋
苗利民
杨建荣
袁华
JI Yefeng;YU Yang;MIAO Limin;YANG Jianrong;YUAN Hua(Jiangsu Key Laboratory of Oral Diseases,Nanjing Medical University,Nanjing 210029,China)
出处
《口腔医学》
CAS
2019年第5期400-403,共4页
Stomatology
基金
国家自然科学基金(81672678
81302361)
江苏高校优势学科建设工程资助项目(2018-87)
江苏省科教强卫工程青年医学人才项目(QNRC2016852)
青蓝工程资助