介孔二氧化硅包覆金纳米双锥近红外光响应药物载体的构建及抗菌性能

Mesoporous silica coated Au nanobipyramids nanoparticle for near-infrared responsive drug delivery system and its application in antibacteria

目的制备介孔二氧化硅包覆金纳米双锥的药物载体,研究其表征、生物相容性、载药性能、近红外光响应性能及抗菌性能。方法运用晶种介导方法合成金纳米双锥,并在其表面包覆介孔二氧化硅,采用扫描电镜、透射电镜和紫外分光光度计对其进行表征,并通过MTT实验检测其生物相容性;观察其米诺环素负载率及近红外光照射对其药物累积释放率的影响。结果成功制备介孔二氧化硅包覆金纳米双锥药物载体,透射电镜下可见无序的孔隙结构,对细胞增殖无明显影响,药物负载率为76.5%,经近红外光照射后药物累积释放量上升41.7%。体外抗菌实验显示显著抑制细菌生长达90%。结论介孔二氧化硅包覆金纳米双锥药物载体具有良好生物相容性、近红外光响应药物释放及抗菌作用,在牙周局部缓控释抗菌中有应用的潜能。

Objective To prepare the drug delivery system of mesoporous silica coated Au nanobipyramids(Au NBPs@SiO2), and study its characterization, biocompatibility, drug loading, near-infrared responsive property and antibacterial property. Methods The Au nanobipyramids(Au NBPs) were synthesized by a seed-mediated method, and coated with mesoporous silica(MSNs). It was characterized by scanning electron microscopy, transmission electron microscopy and ultraviolet spectrophotometer, and its biocompatibility was tested by MTT assay. Then, the effect of its minocycline loading rate and near-infrated light irradiation on the drug accumulation release rate was observed. Results The drug delivery system of Au NBPs@SiO2 was successfully prepared. The disordered pore structure was observed under transmission electron microscope. And it had no obvious effect on cell proliferation. The drug loading efficiency reached 76.5%, and the cumulative drug release increased by 41.7% under NIR irradiation. In vitro antibacterial experiments showed it had significant antibacterial effects, which reached 90%. Conclusion The drug delivery system of Au NBPs@SiO2 has good biocompatibility, near-infrared responsive drug release behavior and antibacterial property, which has application potential in periodontal sustained-release antibacterial therapy.