内生真菌叶下珠生拟茎点霉的次级代谢产物研究（英文）

Cytotoxic Secondary Metabolites from an Endophytic Fungal Strain of Phomopsis phyllanthicola

从广藿香内生真菌叶下珠生拟茎点霉(Phomopsis phyllanthicola A658)中分离纯化得到6个聚酮类化合物,分别鉴定为dothiorelone O(1),2-[3,5-二羟基-2-(7-羟基辛酰基)苯基]乙酸(2),dothiorelone M(3),dothiorelone K(4),cytosporone B(5),和cytosporone C(6).其中化合物1为新化合物,化合物2为新天然产物,新化合物的结构进一步通过HR-ESIMS,1D NMR和2D NMR等光谱技术确定.此外,对分离所得的化合物进行了体外细胞毒测试,结果表明化合物3~5显示出不同程度的细胞毒活性.

The chemical investigation of the broth extract of the endophytic fungus Phomopsis phyllanthicola A658, which was isolated from the medicinal plant Pogostemon cablin, resulted in the isolation of one new compound, named dothiorelone O (1) and one new natural product 2-(3,5-dihydroxy-2-(7-hydroxyoctanoyl)phenyl)acetic acid (2), along with four known derivatives. Their structures were fully characterized by means of detailed spectroscopic analysis for new structures, and in comparison with published data for known compounds. Moreover, all of the compounds were evaluated for in vitro cytotoxic activities against SF-268, MCF-7, HepG-2, and A549 tumor cell lines.