基于多糖组成和含量的3种基原黄精质量比较和识别研究

Quality evaluation and discrimination of Polygonati Rhizoma from three origins based on polysaccharide composition and content

目的通过定性与定量分析,结合化学计量学方法,比较黄精3种基原植物中多糖的组成和含量差异,为黄精药材质量评价提供参考。方法采用红外光谱法(FTIR)和柱前衍生化-HPLC(PMP-HPLC)法比较3种基原黄精中多糖的差异;采用紫外光谱法(UV)测定总多糖含量。结果 3种基原黄精多糖的IR平均光谱和二阶导数图谱差异明显,主成分分析(PCA)、偏最小二乘法判别分析(PLS-DA)及聚类分析(HCA)分析均可将3种基原的黄精加以区分;PMP-HPLC图谱显示3种基原黄精中多糖的单糖组成存在差异,共有成分为D-甘露糖、D-核糖、L-鼠李糖、D-半乳糖醛酸、D-葡萄糖醛酸、D-半乳糖、D-葡萄糖、D-木糖、D-阿拉伯糖和L-岩藻糖;结果表明,所测样品多糖含量符合《中国药典》2015年版的要求。结论本研究所建立方法为黄精类药材的多糖组成研究和质量评价提供了新的参考。

Objective To compare the composition and content of polysaccharides in Polygonati Rhizoma by qualitative and quantitative analysis combined with chemometrics, and provide the reference for the quality evaluation of Polygonati Rhizoma. Methods Fourier transform infrared spectroscopy(FTIR) and pre-column derivatization-HPLC(PMP-HPLC) were employed to reveal the differences of polysaccharide among the three species, and the content of total polysaccharide was determined by ultraviolet spectroscopy(UV). Results The result indicated that the average spectra and the second derivative atlas in FTIR fingerprint of Polygonati Rhizoma was significantly different. The PCA, PLS-DA and HCA analysis provided a further refinement of the method to distinguish three species. Furthermore, the PMP-HPLC showed that the components of monosaccharide and polysaccharides of three species were different. The main common components of the 10 common peaks were identified, which were as follows: D-mannose, D-ribose, L-rhamnose, D-galacturonic acid,D-glucuronic acid, D-galactose, D-glucose, D-xylose, D-arabinose and L-fucose. The content of the total polysaccharide meeted the requirements of Chinese pharmacopoeia 2015 edition Conclusion The study provided a new reference for quality evaluation of P. Rhizoma.