高杯喉毛花中1个新的裂环环烯醚萜苷类化合物

A new secoiridoid glycoside from Comastoma traillianum

目的对藏药高杯喉毛花Comastoma traillianum的化学成分进行研究。方法运用RP-HPLC、柱色谱硅胶、葡聚糖凝胶及RP-C_(18)等多种色谱技术进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构,并用Griess试剂法测定了化合物对脂多糖(LPS)和γ干扰素(IFN-γ)诱导的小鼠单核巨噬细胞RAW 264.7的抗炎活性,并计算半数抑制浓度(IC_(50))值评价其抗炎活性。结果从高杯喉毛花中分离得到了4个裂环环烯醚萜苷类化合物,分别鉴定为高杯喉毛花内脂A(1)、2′-间羟基苯甲酰獐牙菜苷(2)、獐牙菜苦苷(3)、龙胆苦苷(4)。其中化合物1为新化合物,命名为高杯喉毛花内脂A。4个化合物体外抗炎活性测试未显示活性。结论化合物1～4为首次从高杯喉毛花中分离得到,其中化合物1为新化合物。所有化合物在100μmol/L的浓度下均未检测到抗炎活性。

Objective To research the chemical constituents in Comastoma traillianum. Methods The chemical constituents from C.traillianum were isolated and purified by column chromatographic methods of silica gel, RP-HPLC, Sephadex LH-20, and RP-C18.Their structures were elucidated by modern spectroscopy methods, including 1 D and 2 D NMR, HRESIMS, IR, and UV techniques.The anti-inflammatory activity of compounds against LPS and IFN-γ-induced mouse RAW 264.7 macrophages was determined by Griess, and the IC50 value was calculated to evaluate their anti-inflammatory activity. Results Four secoiridoid glycosides(1-4)were isolated from C. traillianum, and identified as comtraide A(1), deacetylcentapicrin(2), swertiamarin(3), and gentiopieroside(4).Compound 1 is a new compound named comtraide A. All compounds were tested for their in vitro anti-inflammatory activity and no activity was found. Conclusion Compounds 1-4 are isolated for the first time from C. traillianum, and compound 1 is a new compound. However, no significant anti-inflammatory activities were detected in all these compounds at concentrations up to 100μmol/L.