ABCG2基因多态性与结直肠癌发生发展的相关性研究

Study on the correlation between the polymorphism of ABCG2 gene and the occurrence and progression of colorectal cancer

目的探讨ABCG2基因rs2231137和rs2231142单核苷酸多态性(SNP)与结直肠癌发生发展的关系。方法选取2016年1月至2017年7月采用病例-对照研究,收集结直肠癌患者外周血样本690份,社区健康体检者外周血样本431份,使用TaqMan实时荧光定量PCR方法进行基因分型,应用χ~2检验和logistic回归进行ABCG2 rs2231137和rs2231142基因多态性与结直肠癌关联分析。结果对性别和年龄进行分层分析显示,携带ABCG2 rs2231142 AA基因型的男性,与CC基因型相比,结直肠癌发病风险降低(OR=0.50,95%CI:0.28～0.90,P=0.022)。年龄≤60岁携带rs2231137 AA基因型的结直肠癌患者发生Ⅰ、Ⅱ期肿瘤的比例高于Ⅲ、Ⅳ期(OR=0.29,95%CI:0.14～0.61,P=0.001)。结论 ABCG2 rs2231137和rs2231142单核苷酸多态性与结直肠癌的发生发展相关。

Objective The aim of the present study was to explore the correlation between the single nucleotide polymorphisms(SNP) of ABCG2 gene rs2231137 and rs2231142 and the occurrence and progression of colorectal cancer. Methods From January 2016 to July 2017, a case-control study was conducted to collect 690 peripheral blood samples from patients with colorectal cancer, and 431 peripheral blood samples from community healthy individuals.TaqMan real-time fluorescence quantitative PCR was used for genotyping.Chi-square test and logistic regression were used to analyze the association of ABCG2 rs2231137 and rs2231142 gene polymorphism with colorectal cancer. Results By stratified analysis of sex and age, it showed that men with the ABCG2 rs2231142 AA genotype had lower risk of colorectal cancer compared with the CC genotype(OR=0.50, 95%CI:0.28-0.90, P=0.022).The percentage of patients with stage Ⅰ and stage Ⅱ tumors was 0.293 times higher than those with stage Ⅲ and Ⅳ tumors among patients aged 60 years and older who carried rs2231137 AA genotype, suggesting a slower progression of tumor in those patients(OR=0.29, 95%CI:0.14-0.61, P=0.001). Conclusions Single nucleotide polymorphisms of rs2231137 and rs2231142 in ABCG2 gene were associated with the occurrence and development of colorectal cancer.