人口腔鳞状细胞癌细胞系TSCC2016的建立及生物学特性分析

Establishment of a new cell line of oral squamous cell carcinoma cell: TSCC2016

目的:建立一株口腔鳞状细胞癌细胞系TSCC2016,明确其生物学特性,为研究口腔鳞状细胞癌的发病机制和临床治疗提供工具。方法:选取新鲜的口腔鳞状细胞癌患者手术标本,采用组织块培养法,建立口腔鳞状细胞癌细胞系TSCC2016,观察其细胞形态和生长特性,对其表面标记、细胞核型和裸鼠成瘤等进行检测。采用SPSS13.0软件包对数据进行统计学分析。结果:TSCC2016的传代已经超过100代。细胞生长稳定,形态为均一的多角形上皮细胞,具有典型的口腔鳞状细胞癌细胞特点。细胞STR分型结果表明,TSCC2016细胞为原代培养出的口腔鳞状细胞癌细胞,无其他肿瘤细胞系污染。TSCC2016细胞的成瘤性较好,成瘤率为100%,瘤体生长较快。结论:成功建立了一株口腔鳞状细胞癌细胞系TSCC2016,为口腔鳞状细胞癌的基础研究提供了一个稳定的细胞株。

PURPOSE: To establish a new cell line of oral squamous cell carcinoma(OSCC), and explore its biological characteristics. METHODS: Fresh tumor tissue from a female patient, which was pathologically confirmed to be oral squamous cell carcinoma(OSCC), was collected during surgery. Tissue sample was cut into small pieces and cultured in nutrient solution. A new cell line of oral squamous cell carcinoma(TSCC2016) was established, and its cellular morphology was described. Cell proliferation, epithelial cell differentiation, tumorigenicity, and cell karyotype were examined. The data were analyzed with SPSS13.0 software package. RESULTS: TSCC2016 was successfully established and subcultured for more than 100 passages. TSCC2016 cells had characteristics of polygonal epithelial cell, epithelial cell differentiation, and good tumorigenicity. STR analysis showed that TSCC2016 cells were primarily cultured OSCC cells without any other cell line contamination. CONCLUSIONS: The new cell line of OSCC cell line(TSCC2016) is a stable cell line for further basic research.