微小RNA-181a-5p、320a及其靶基因TGF-β1在食管鳞癌中的表达

Expression of miR-181a-5p, miR-320a and TGF-β1 in Esophageal Squamous Cell Carcinoma

目的:检测人食管鳞癌组织中miR-181a-5p、miR-320a及TGF-β1的表达情况,研究其在食管鳞癌发生中的作用。方法:收集食管鳞癌及癌旁正常组织各3例,抽取总RNA,利用miRNA芯片技术检测miRNA的表达;采用qRT-PCR方法验证miRNA芯片结果的可靠性。采用软件和数据库对差异表达miRNA调控的靶基因进行初步分析。收集食管鳞癌及对照正常组织各22例,采用qRT-PCR方法检测miR-181a-5p、miR-320a和TGF-β1mRNA。结果:miRNA芯片结果显示,共有22种miRNA在食管鳞癌和正常组织中的表达差异有统计学意义(P<0.05),其中6种显著上调,16种显著下调;qRT-PCR证实miR-320a表达下降,miR-181a-5p表达上升,差异均有统计学意义(P<0.05),与芯片结果一致;TGF-β1mRNA在食管鳞癌中表达较对照正常组织稍升高,但差异无统计学意义。结论:miR-181a-5p和miR-320a可能参与食管鳞癌的发生发展过程。

Objective: To investigate the expression of miR-181a-5p, miR-320a and TGF-β1 in esophageal squamous cell carcinoma and study their roles in the development of esophageal squamous cell carcinoma. Methods: Three samples of esophageal squamous cell carcinoma and adjacent normal tissue were collected. The miRNA array was used to detect the expression of miRNA. The reliability of the miRNAs chip results was verified using real-time quantitative polymerase chain reaction ( qRT-PCR). Differentially expressed miRNA-regulated target genes were analyzed by software and database. miR-181a-5p, miR-320a and TGF-β1, extracted from 22 cases of esophageal squamous cell carcinoma and 22 cases of normal tissue, were verified using qRT-PCR. Results: The miRNA chip results showed that 22 miRNAs differentially expressed in esophageal squamous cell carcinoma compared with those in normal tissue ( P < 0. 05 ). Six miRNAs significantly increased, while 16 significantly decreased. It was confirmed that results of qRT-PCR were consistent with those of the miRNA array. qRT-PCR confirmed that the expression of miR-320a decreased and that of miR-181a-5p increased in esophageal squamous cell carcinoma compared with those in normal tissue ( P < 0. 05 ). The expression of TGF-β1 in esophageal squamous cell carcinoma was slightly higher than that in normal tissue ,but the difference was not statistically significant. Conclusion: miR-181a-5p and miR-320a may be engaged in carcinogenesis of esophageal squamous cell carcinoma.