灵芝蛛网病病原菌及其生物学特性

Identification and biological characteristics of Cladobotryum mycophilum causing cobweb disease on Ganoderma lingzhi

为明确吉林省蛟河市灵芝Ganoderma lingzhi栽培主产区发生的疑似灵芝蛛网病的病原菌,作者通过罹病灵芝子实体病原物的分离纯化、致病性测定、形态学和分子生物学鉴定以及病原菌的生物学特性研究,证明引起吉林省蛟河市灵芝蛛网病的病原菌为嗜菌枝葡霉Cladobotryum mycophilum。该菌营养体最适生长条件为温度25°C、pH 5、蔗糖作碳源、酵母浸粉作氮源,光照对菌丝体生长有一定的抑制作用,完全黑暗最适宜生长。本文研究结果为进一步研究该病害的发生规律和防治措施提供了理论参考。

In 2018, the alleged cobweb disease was found in Ganoderma lingzhi-growing farm at Jiaohe, Jilin Province. Morphological identification and molecular analysis of ITS DNA sequence proved that the pathogen was Cladobotryum mycophilum. Artificial inoculation confirmed that the pathogen infected fruitbodies of Ganoderma lingzhi. Its mycelia grew favorably under the conditions of 25℃ and pH 5 on medium using glucose as carbon source and yeast extract as nitrogen source. Light might inhibit mycelial growth to some extent,while dark stimulated mycelial growth. The results provide a useful reference for effective control of the cobweb disease.