7360例呼吸道感染儿童病毒抗原七联检的临床结果分析

Clinical analysis of 7 360 children with respiratory virus infection

目的 通过对四川大学华西第二医院就诊的7360例急性呼吸道感染患儿进行呼吸道病毒抗原检测,分析不同病毒在急性呼吸道感染中的分布情况,为儿童呼吸道感染的早期诊治提供流行病学依据。方法 提取患儿鼻咽拭子,采用直接免疫荧光法(DFA)检测7种呼吸道病毒抗原,包括流感病毒A型(FluA)、流感病毒B型(FluB)、副流感病毒1型(PIV1)、副流感病毒2型(PIV2)、副流感病毒3型(PIV3)、呼吸道合胞病毒(RSV)和腺病毒(ADV)。结果 7360例急性呼吸道感染患儿中,检出呼吸道病毒783例,检出率为10.64%。其中检出率最高的是RSV545例(7.40%),其次分别为PIV3107例(1.45%),ADV62例(0.84%),FluB25例(0.34%),FluA24例(0.33%),PIV118例(0.24%),混合型8例(0.11%),PIV22例(0.03%)。结论 7项呼吸道病毒检测可以明确引起患儿急性呼吸道感染的病原体,为临床用药提供合理诊断依据,对临床药物合理使用具有指导意义。

Objective To analyze the distribution of different viruses in acute respiratory tract infections by detecting respiratory viral antigens in 7 360 children with acute respiratory tract infections in West China Second Hospital of Sichuan University,in order to provide epidemiological basis for early diagnosis and treatment of children's respiratory tract infections. Methods Nasopharyngeal swabs were extracted and 7 respiratory viruses were detected by direct immunofluorescence (DFA),including influenza A (Flu A),influenza B (Flu B),parainfluenza type 1 (PIV1),parainfluenza virus type 2 (PIV2),parainfluenza virus type 3 (PIV3),respiratory syncytial virus (RSV) and adenovirus (ADV). Results Among 7 360 children in this study,783 cases of respiratory virus were detected,and the detection rate was 10.64%.The highest detection rate was 545 cases ( 7.40 %) of RSV,followed by 3 107 cases of PIV (1.45%),62 cases of ADV (0.84%),25 cases of FluB ( 0.34 %),24 cases of Flu A (0.33%),18 cases of PIV1 (0.24%),8 cases of mixed type (0.11%) and 2 cases of PIV2 (0.03%). Conclusion Seven-respiratory-virus test can identify the pathogens causing acute respiratory infection in children,provide a reasonable diagnostic basis for clinical use,and have guiding significance for the rational use of clinical drugs.