冠突曲霉Fig基因敲除菌株的构建及表型分析

Construction and Phenotypic Analysis of Gene Knocking Strain of Fig Gene in Aspergillus cristatus

为了研究冠突曲霉(Aspergillus cristatus) Fig基因的功能,本研究采用同源重组的方法获得敲除株。并对敲除株进行功能验证。结果显示对Fig基因敲除株培养观察并与野生型菌株相比发现,在含有不同浓度NaCl的MYA培养基中,仍能产生成熟的子囊孢子和分生孢子,但分生孢子数量是野生型的1/4,子囊孢子数量是野生型的1/5,且敲除株菌落几乎没有渗出液,无皱褶,菌落表面干燥,色素产生量急剧下降,这些结果表明Fig基因对冠突曲霉产孢起正调控,本实验为冠突曲霉发育调控机制的研究提供了一定的技术基础。

In order to study the function of the Fig gene of Aspergillus cristatus, this study used homologous recombination to obtain knockout strain. Functional verification of knockout strain was performed. The results showed that the culture of the Fig knockout strain was observed and compared with the wild type strain, it was found that mature ascospores and conidia were still produced in the MYA medium containing different concentrations of NaCl, but the number of conidia was 1/4 of the wild type, the number of ascospores was 1/5 of the wild type, and the colony of the knockout strain had almost no exudate, and there was no wrinkle, the surface of the colony was dry, and the amount of pigment production droped sharply. These results indicated that the Fig gene was crowned, the sporulation of Aspergillus cristatus was positively regulated. This experiment provided a certain technical basis for the study of the regulation mechanism of Aspergillus cristatus development.