人PRMT5基因的生物信息学分析

Bioinformatic Analysis of Human PRMT5 Gene

PRMT5 (Protein arginine methyltransferase 5)已经广泛地作为组蛋白甲基转移酶修饰H4R3、H2AR3和H3R8,从而潜在地影响多个信号传导途径。本研究为了对人PRMT5基因进行生物信息学方面的深入分析,以PRMT5基因序列及编码蛋白序列为材料,通过生物信息学方法分析了PRMT5基因的DNA序列、启动子及CpG岛、RNA结构,及该蛋白的理化性质、亚细胞定位、信号肽与跨膜区域、互作蛋白、系统发育等。研究结果表明,人PRMT5在物种间的保守性相对较高,位于染色体14q11.2,大小为1 911 bp,潜在核心启动子在1 014~1 064 bp,没有预测到CpG岛。PRMT5基因编码637个氨基酸残基,分子量为10 157 Da,等电点为5.88,不稳定系数是44.33;该蛋白更可能定位于细胞质,无明显的信号肽及跨膜结构;主要的二级结构元件是α-螺旋结构和无规卷曲,包含一个SAM-dependent MTase功能结构域,同时有10个可能的互作蛋白;进化分析表明黑猩猩和猕猴与人PRMT5蛋白亲缘关系最近。

PRMT5(Protein Arginine Methyltransferase 5) had been extensively characterized as a histone methyltransferase that modifies H4 R3, H2 AR3 and H3 R8, and affects potential multiple signaling pathways. In this research, the nucleotide and encoded protein sequence of PRMT5 were used as materials in order to further analyze bioinformatics of human PRMT5 gene. The nucleic acid sequence and amino acid sequence of PRMT5 gene were analyzed and predicted by bioinformatics approaches, including DNA sequence, promoter and CpG island and RNA structure, physicochemical properties, the subcellular position, signal peptide, transmembrane region, protein interaction networks and evolutionary tree. The results showed that PRMT5 are relatively conserved among species, located on chromosome 14 q11.2, with a size of 1 911 bp. The potential core promoter is located at 1 014~1 064 bp and no CpG island was predicted. The PRMT5 gene encodes 637 amino acid residues with a molecular weight of 10 157 Da, an isoelectric point of 5.88 and an instability coefficient of44.33. The protein is more likely localized in the cytoplasm with no obvious signal peptide and transmembrane structure. The major secondary structure elements are an α-helical structure and a random coil, which contains a SAM-dependent MTase functional domain with 10 possible interacting proteins. Phylogenetic analysis shows that Chimpanzees and Macaques have the closest relationship with human PRMT5 protein.