SC-CO2和乙酸乙酯萃取芸香中活性成分的比较分析

Comparative Analysis on Extraction of Active Components of Ruta graveolens by Supercritical CO2 and Ethyl Acetate

为了探讨超临界二氧化碳(supercritical carbon dioxide, SC-CO2)技术与提取物的分级分离在萃取芸香活性成分的应用价值,本研究采用SC-CO2和乙酸乙酯萃取芸香中植物蜡和活性成分,并调查粒径和CO2流量对提取产量的影响。在250 bar、40℃条件下提取,并使第一个分离器冷却到-10℃,可获得较好的提取效率。当粒径较小时,提取过程更快,即内部传质控制该过程。分级分离可选择性去除表皮植物蜡,约占由SC-CO2处理产生的总提取物的77.5%W/W。第二分离器中的获得的提取物中活性化合物可达86.3%W/W。随后采用气相色谱-质谱联用仪(gas chromatography-mass spectrometry, GC-MS)分析表明,乙酸乙酯提取物低于SC-CO2提取物的萃取效率,主要是由于提取物中含有大量的植物蜡。本研究为超临界二氧化碳技术在萃取芸香活性成分方面的提供技术参考。

In order to investigate the application of supercritical carbon dioxide(SC-CO2) technology and fractionation of extracts in the extraction of the active components of Ruta graveolens. In this study, plant wax and active components of Ruta graveolens were extracted by SC-CO2 and ethyl acetate, and the effects of particle size and CO2 flute rate on extraction yield were investigated. Extraction at 250 bar, 40℃ and cooling of the first separator to-10℃ resulted in better extraction efficiency. When particle size was small, the extraction process was faster and internal mass transfer controlled the process. Fractionation selectively removed epidermal waxes,accounting for about 77.5% W/W of total extract produced by SC-CO2 treatment. The active compound in the extract obtained in the second separator amounts to 86.3% W/W. Subsequent analysis by Gas Chromatography-Mass Spectrometry(GC-MS) showed that the extraction efficiency of the extract using ethyl acetate was lower than that of the SC-CO2 extract. Mainly because the extract contains a lot of vegetable wax. This study is a technical theoretical reference for the extraction of musk active ingredients by supercritical carbon dioxide technology.